000			02730nam a22003617a 4500
001			G94564
003			MX-TxCIM
005			20220708180532.0
008			220708s2010 mx ||||| |||| 10| 0 eng d
020			_a978-979-1159-41-8
040			_aMX-TxCIM
041			_aeng
090			_aCIS-6129
100	0		_aShibin Gao _98403
245	1	0	_aRevisiting the hetero-fertilization phenomenon in maize using molecular markers
260			_aMexico, DF (Mexico) : _bCIMMYT, _c2010.
520			_aWe have developed a seed DNA-based marker-assisted selection (MAS) system using single maize seeds. The accuracy of MAS based on this method may be affected by hetero-fertilization (HF), which may cause seed DNA-based analysis to wrongly predict plant genotypes due to genotyping of endosperm DNA which could be different from that of the embryo and thus the derived plant. To investigate the potential influence of HF on seed DNAbased MAS and the effect of different genetic backgrounds, five F2 and four three-way segregating populations (each with 534 to 1024 individuals) were used for comparative SSR marker-based analysis of endosperm and embryo. The results indicate that the frequency of HF within these populations ranged from 0.14% to 2.18%, with an average of 1.16%. The highest proportion of HF across both types of population was contributed by the pollen gametes, as reported previously based on morphological marker analyses. This is the first report of HF contributed by maternal gametes in populations from three-way crosses, albeit at very low frequency (0.14%-0.65%). Although single fertilization or haploid tissues were not observed in the tested materials, seed DNA-based analysis combined with specific genetic stocks could be useful for revealing the relationships among various aberrant fertilization phenomena.
536			_aGenetic Resources Program|Global Maize Program
546			_aText in English
594			_aINT3336|INT2735
650		7	_aFertilization _2AGROVOC _98695
650		7	_aGenotyping _2AGROVOC _922057
650		7	_aGenetic markers _2AGROVOC _91848
650		7	_aMarker-assisted selection _2AGROVOC _910737
650		7	_aMaize _2AGROVOC _91173
700	1		_aMartinez, C. _924854
700	1		_aKrivanek, A.F. _96435
700	1		_a Crouch, J.H. _95274
700	1		_9857 _aYunbi Xu _gGlobal Maize Program _8INT2735
711	2		_96553 _aAsian Regional Maize Workshop _n(10th : _dOctober 20-23, 2008 : _cMakassar, Indonesia)
773			_dMexico : CIMMYT, 2010. _gp. 197-201 _tAsian Regional Maize Workshop, 10. Maize for Asia - Emerging Trends and Technologies; Proceedings of The Asian Regional Maize Workshop; Makassar (Indonesia); 20-23 Oct. 2008 _wG94189 _z978-979-1159-41-8
942			_cBP _2ddc _n0
999			_c7983 _d7983