000			02989nam a22004577a 4500
001			G81203
003			MX-TxCIM
005			20211006084758.0
008			121211s ||||f| 0 p|p||0|| |
020			_a970-648-116-8
040			_aMX-TxCIM
072		0	_aF30
072		0	_aH20
090			_aLook under series title
100	1		_aKasim, F. _uAsian Regional Maize Workshop, 8: New Technologies for the New Millennium
110	0		_aCentro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT), Mexico, DF (Mexico)
111	2		_aProceedings of the Asian Regional Maize Workshop _cBangkok (Thailand) _d5-8 Aug 2002
245	0	0	_aPreliminary marker assisted selection breeding program for downy mildew resistance in Indonesia
260			_aMexico, DF (Mexico) _bCIMMYT : _c2004
300			_ap. 82-90
340			_aPrinted
520			_aThe systemic disease of downy mildew (DM) caused by Peronosclerospora maydis Shaw has been an important limiting factor for maize production in Indonesia. Resistance against P. maydis is necessary for developing high yielding cultivars to be released in the country. The molecular marker assisted selection (MAS) program for breeding resistance against P. maydis started in 1999 when Indonesia joined the Asian Maize Biotechnology Network (AMBIONET). A set of inbred lines was phenotypically evaluated in four sites for DM under artificial inoculation using spreader row technique. Several lines showed consistent resistance reaction, including Ki3, P345G/S2B46-2-2-1-2-B-B-B, Nei 9008, AMATL COHS-115-1-2-3-3-1-2-B-B, AMATL COHS-9-1-1-1-1-1-2-3, and Nei 9202. Four of the lines that are highly susceptible were then used to trap the pathogen under natural condition at four hotspot areas. Molecular analysis of the DM by single strand conformational polymorphism (SSCP) using Peronosclerospora genus-specific (PGS) primers did not successfully differentiate DM samples from each location. This result was confirmed with RFLP analysis. A mapping population was developed using two lines: J1-46-2-2-3 f, an elite inbred which developed by InCRI but susceptible to DM as recurrent parent, and AMATL COHS-9-1-1-1-1-1-2-3, a resistant CIMMYT inbred as donor parent. SSR analysis of six inbred lines using 112 primers revealed high level of polymorphism between JI-46-2-2-3f and AMATL COHS-9-1-1-1-1-1-2-3.
546			_aEnglish
591			_a0501|AGRIS 0501|AL-Maize Program
593			_aJuan Carlos Mendieta
595			_aCPC
650	1	7	_aBreeding _gAGROVOC _2 _91029
650	1	0	_aIndonesia _91157
650	1	7	_aMaize _gAGROVOC _2 _91173
650	1	0	_aMildews
650	1	0	_aPeronosclerospora
650	1	0	_aPest control
650	1	7	_aPlant diseases _gAGROVOC _2 _91206
650	1	0	_aSeed production _91253
653	0		_aCIMMYT
700	1		_aAzrai, M., _ecoaut.
700	1		_aRuswandi, D., _ecoaut.
700	1		_aSrinivasan, G.|Zaidi, P.H.|Prasanna, B.M.|Gonzalez, F.|Lesnick, K. _eeds.
700	1		_aSutrisno, D., _ecoaut.
942			_cPRO
999			_c7444 _d7444