000 | 03709nam a22005657a 4500 | ||
---|---|---|---|
001 | G80103 | ||
003 | MX-TxCIM | ||
005 | 20250211163013.0 | ||
008 | 121211s ||||f| 0 p|p||0|| | | ||
020 | _a970-648-120-6 | ||
040 | _aMX-TxCIM | ||
072 | 0 | _aF30 | |
072 | 0 | _aH10 | |
090 | _aCIS-4174 | ||
110 | 0 | _aCentro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT) Kenya | |
100 | 1 |
_aHearne, S. _gGenetic Resources Program _gDG's Office _8INT3287 _9912 |
|
245 | 0 | 0 | _aTransposons and tolerance; teh identification of genes for Striga tolerance in maize |
260 |
_aNairobi (Kenya) _bKARI*CIMMYT : _c2002 |
||
300 | _ap. 173 | ||
340 | _aPrinted | ||
500 | _aAbstract only | ||
520 | _aStriga is one of the most severe constraints to cereal production in areas of the semi-arid tropics of Africa where subsistence agriculture is predominant. The development of Striga tolerant or resistant germplasm has been the goal of many maize breeders. However, to date, no resistant maize has been developed and tolerance, though improved, is still limited. Funded by the Rockefeller Foundation, we developed a large population of maize which contained transposable element-induced mutations. The transposon selected was the mutator element system. Mutator elements preferentially insert into coding regions of the genome and are therefore optimal for transposon-tagging. 8000 F2 families from the transposon-tagged maize population were screened in the field in Kibos during 1 998 and 1999. Interesting families were identified as those which had segregating Striga-free plants (no emergence) within the family. Twenty three families have been identified which have no/low emergence of Striga. All these families displayed 1:3 segregation for the Striga free trait (25% Striga free, 75% Striga emergence), this indicates that a single recessive mutation is responsible for the observed phenotype. The progeny of one of these families has been screened in pot experiments in the laboratory and the phenotype observed in the field has been confirmed. The lack of Striga emergence was not due to altered germination stimulant production or perturbed attachment. However, the biomass of the parasite on this transposon-tagged line was much lower than that on susceptible control lines. As such the growth of Striga attached to the transposon-tagged plants was severely impaired resulting in a low incidence of emergence. Work is currently underway to: develop molecular markers to aid in the introgression of this trait into other maize lines; to clone and identify the gene underlying the trait; to investigate the physiological basis of Striga growth retardation and to; evaluate other interesting maize families in the laboratory. | ||
536 | _aConservation Agriculture Program|Genetic Resources Program | ||
546 | _aEnglish | ||
591 | _a0409|AGRIS 0401|AL-Maize Program | ||
593 | _aJuan Carlos Mendieta | ||
594 | _aINT3287|INT2340 | ||
595 | _aCSC | ||
650 | 1 | 0 | _aAngiosperms |
650 | 1 | 0 |
_91897 _aBiomass _gAGROVOC |
650 | 1 | 0 | _aInfestation |
650 | 1 | 7 |
_aMaize _gAGROVOC _2 _91173 |
650 | 1 | 0 |
_91195 _aParasitic plants _gAGROVOC |
650 | 1 | 0 | _aPest control |
650 | 1 | 0 | _aStriga hermonthica |
650 | 1 | 0 | _aTripsacum |
653 | 0 | _aCIMMYT | |
653 | 0 | _aKARI | |
700 | 1 | _aFriesen, D.K.|Palmer, A.F.E. | |
700 | 1 |
_aGrimanelli, D., _ecoaut. |
|
700 | 1 |
_aGurney, A.L., _ecoaut. |
|
700 | 1 |
_aHoisington, D.A., _ecoaut. |
|
700 | 1 |
_aKanampiu, F.K., _ecoaut. |
|
700 | 1 |
_aMbogo, P.O., _ecoaut. |
|
700 | 1 |
_aOdhiambo, G.D., _ecoaut. _9628 |
|
700 | 1 |
_aPress, M.C., _ecoaut. |
|
700 | 1 |
_aScholes, J.D., _ecoaut. |
|
700 | 1 |
_aVasey, R., _ecoaut. |
|
942 | _cPRO | ||
999 |
_c7333 _d7333 |