000 02488nam a22004817a 4500
001 80096
003 MX-TxCIM
005 20170719154809.0
008 121211s ||||f| 0 p|p||0|| |
020 _a970-648-120-6
040 _aMX-TxCIM
072 0 _aA50
072 0 _aF01
090 _aCIS-4170
100 1 _aWarburton, M.
_uIntegrated Approaches to Higher Maize Productivity in the New Millennium
110 0 _aCentro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT) Kenya
111 2 _a7. Proceedings of the Eastern and Southern Africa Regional Maize Conference
_cNairobi (Kenya)
_d5-11 Feb 2002
245 0 0 _aUse of molecular markers in maize diversity studies at CIMMYT
260 _aNairobi (Kenya)
_bKARI|CIMMYT :
_c2002
300 _ap. 130-133
340 _aPrinted
520 _aFingerprinting and genetic diversity laboratory protocols have been optimized for high-throughput analysis of maize. Eighty-five SSR markers have been chosen that span the entire genome and can be multiplexed (4-12 SSRs per lane) on an automatic DNA sequencer. Maize populations can also be fingerprinted in a very efficient method by bulking 15 individuals from the same population; this bulk is amplified and run together on the sequencer. This allows the use of peak area from the sequencer output to estimate allele frequencies from each population. Two bulks per population are fingerprinted to have a total of 30 individuals per population in the estimation. Only a limited structure can be deduced in the inbred lines by using molecular markers, reflecting the breeding strategy at CIMMYT of mixing many germplasm pools to increase diversity in tropical and subtropical breeding lines. Origin, and to a lesser extent heterotic groupings, of the populations are reflected in the SSR diversity measurements.
546 _aEnglish
591 _a0409|AGRIS 0401|AL-Maize Program
593 _aJuan Carlos Mendieta
595 _aCSC
650 1 0 _aDNA
650 1 0 _aGenomes
_91131
650 1 0 _aInbred lines
_91155
650 1 0 _aMaize
_91173
650 1 0 _aMolecular genetics
653 0 _aCIMMYT
653 0 _aKARI
650 1 0 _91203
_aPlant breeding
_gAGROVOC
650 1 0 _91006
_aAgricultural research
_gAGROVOC
700 1 _aAmbriz, S.,
_ecoaut.
700 1 _aDiaz, L.,
_ecoaut.
700 1 _aFriesen, D.K.|Palmer, A.F.E.
700 1 _aHoisington, D.A.,
_ecoaut.
700 1 _aVillordo, E.,
_ecoaut.
700 1 _aXianchun Xia,
_ecoaut.
_9377
942 _cPRO
999 _c7329
_d7329