000 03614nam a22005177a 4500
001 G80093
003 MX-TxCIM
005 20220104230314.0
008 121211s ||||f| 0 p|p||0|| |
020 _a970-648-120-6
040 _aMX-TxCIM
072 0 _aF01
072 0 _aH10
090 _aCIS-4169
100 1 _aSonga, J.M.
_uIntegrated Approaches to Higher Maize Productivity in the New Millennium; 7. Proceedings of the Eastern and Southern Africa Regional Maize Conference; Nairobi (Kenya); 5-11 Feb 2002
110 0 _aCentro Internacional de Mejoramiento de Maiz y Trigo (CIMMYT) Kenya
245 0 0 _aMass rearing of the maize stem borers Chilo partellus, Busseola fusca, Sesamia calamistis, Chilo orichalcociliellus and Eldana saccharina at kari, Katumani
260 _aNairobi (Kenya)
_bKARI|CIMMYT :
_c2002
300 _ap. 120-124
340 _aPrinted
520 _aAn insect rearing facility was established at the Kenya Agricultural Research Institute (KARI) Centre of Katumani in Machakos, Kenya, in 1999, and has to date improved significantly in its scale of operation. The primary purpose of this facility is to provide stem borers for use in resistance screening studies, insect bioassays and for oviposition/feeding preference studies within KARI projects. The stem borers that are reared are: Chilo partellus (Swinhoe), Chilo orichalcocilliellus Strand, Eldana saccharina Walker (Lepidoptera: Pyralidae) Busseola fusca Fuller and Sesamia calamistis Hampson (Lepidoptera: Noctuidae), with the bulk production being for the first two species due to their relative higher demand for use in resistance screening studies. This paper describes the insect rearing facility, procedures used, records taken, problems encountered during rearing and the steps taken to address them. Since initiation of the insectary in 1999, the scale of production has improved significantly, with the seasonal supply of insects increasing from 26,000 in long rains 2000, to 961,689 stem borers in the long rains 2001. There have also been improvements in the quality of production, record keeping and in the supply system in terms of synchrony of the stem borer supply with the various end-uses. As part of the efforts to improve the rearing techniques, a laboratory study was conducted to compare the survival to pupation of B. fusca larvae in large plastic jars (16 x 7.5 cm -250 ml of diet -20 larvae) compared to glass vials (7.5 x 2.5 cm -15ml of diet -1 larva). There was no significant difference in the survival to pupation of larvae reared in the plastic containers (80.5%) and the glass vials (85.2%) (t= 1.59; d.f = 10; P= 0.143). Since the plastic container saves on the time of infestation (egg batches can be used), is less breakable and is cheaper, it has now been adopted for the rearing of B. fusca at the Katumani insectary.
536 _aGlobal Maize Program
546 _aEnglish
591 _a0409|AGRIS 0401|AL-Maize Program
593 _aJuan Carlos Mendieta
594 _aINT2460
595 _aCSC
650 1 0 _aBusseola
650 1 0 _aChilo partellus
650 1 0 _aFeeding
650 1 0 _aInsect control
_91162
650 1 0 _aLepidoptera
650 1 7 _aMaize
_gAGROVOC
_2
_91173
650 1 0 _aNoctuidae
650 1 0 _aOviposition
650 1 0 _aPyralidae
650 1 0 _aSesamia calamistis
650 1 0 _aStem borer
650 1 0 _aTechnology
_gAGROVOC
_91988
653 0 _aCIMMYT
653 0 _aKARI
700 1 _aBergvinson, D.J.,
_ecoaut.
700 1 _aFriesen, D.K.|Palmer, A.F.E.
700 1 _aMugo, S.N.
_gFormerly Global Maize Program
_8INT2460
_9840
942 _cPRO
999 _c7328
_d7328