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022 _a2095-3119
022 _a2352-3425 (Online)
024 8 _ahttps://doi.org/10.1016/j.jia.2024.04.033
040 _aMX-TxCIM
041 _aeng
100 0 _aJianqi Zeng
_921712
245 1 0 _aFine mapping and candidate gene analysis of a major QTL for grain length on chromosome 5BS in bread wheat
260 _aChina :
_bElsevier B.V. ;
_bKeAi Communications Co.,
_c2025.
500 _aPeer review
500 _aOpen Access
520 _aLarge grain is a favorable trait for appearance quality and a large sink potential in wheat breeding. The stable QTL QGl.caas-5BS for grain length was previously identified in a recombinant inbred line population from the cross of Zhongmai 871 (ZM871) and its sister line Zhongmai 895 (ZM895). Here, a BC1F6 residual heterozygous line was selected from the cross of a ZM871/ZM895//ZM871 population, and six heterozygous recombinant plants were identified in the BC1F7 population from self-pollination of the heterozygous line. QGl.caas-5BS was delimited into an interval of approximately 2.2 Mb flanked by markers Kasp_5B33 and Kasp_5B2 (25.3–27.5 Mb) by phenotyping and genotyping the secondary mapping populations derived from these heterozygous recombinant plants. Five genes were predicted as candidates of QGl.caas-5BS based on sequence polymorphism and differential expression analyses. Further mutation analysis showed that TraesCS5B02G026800 is likely the causal gene of QGl.caas-5BS. The gene-specific marker Kasp_5B_Gl for TraesCS5B02G026800 was developed, and a significant genetic effect of QGl.caas-5BS on grain length was identified in a validation population of 166 cultivars using this marker. These findings lay a good foundation for map-based cloning of QGl.caas-5BS and provide a breeding-applicable marker for the improvement of grain length in wheat.
546 _aText in English
597 _dNational Natural Science Foundation of China
_dChinese Academy of Agricultural Sciences (CAAS)
_dKey Research and Development Program of Xinjiang Uygur Autonomous Region
_dHebei Provincial Natural Science Foundation
650 7 _aBreeding
_2AGROVOC
_91029
650 7 _aCandidate genes
_2AGROVOC
_929589
650 7 _aForecasting
_2AGROVOC
_92701
650 7 _aGrain
_2AGROVOC
_91138
650 7 _aTriticum aestivum
_2AGROVOC
_91296
650 7 _aQuantitative Trait Loci
_2AGROVOC
_91853
700 0 _aDehui Zhao
_91854
700 0 _aLi Yang
_91852
700 0 _aYufeng Yang
_940698
700 0 _aDan Liu
_919678
700 0 _aYubing Tian
_91856
700 0 _aFengju Wang
_911073
700 0 _aShuanghe Cao
_95093
700 0 _aXianchun Xia
_9377
700 1 _aHe Zhonghu
_gGlobal Wheat Program
_8INT2411
_9838
700 0 _aYong Zhang
_91857
773 0 _tJournal of Integrative Agriculture
_dChina : Elsevier Ltd. ; KeAi Communications Co., 2025.
_x2095-3119
_gv. 24, no. 7, p. 2465-2474
_wu56517
856 4 _yOpen Access through DSpace
_uhttps://hdl.handle.net/10883/36189
942 _cJA
_n0
_2ddc
999 _c69606
_d69598