000			03658nab|a22004817a|4500
001			69603
003			MX-TxCIM
005			20251126092200.0
008			2511252025|||||cc ||p|op||||00||0|eng|dd
022			_a2095-5421
022			_a2214-5141 (Online)
024	8		_ahttps://doi.org/10.1016/j.cj.2025.06.002
040			_aMX-TxCIM
041			_aeng
100	0		_aSiyang Liu _937244
245	1	0	_aTaIAA10-6D orchestrates processing quality and grain yield by modulating glutenin/gliadin ratio and plant morphogenesis in wheat
260			_aChina : _bElsevier B.V. ; _bKeAi Communications Co. Ltd., _c2025.
500			_aPeer review
500			_aOpen Access
520			_aHigh molecular weight glutenin subunits (HMW-GS), major components of seed storage proteins in wheat, have large effects on processing quality. GLU-1 genes encode HMW-GS and their expression is mainly controlled at the transcriptional level by interactions between cis-regulatory elements and transcription factors. We previously identified an Aux/IAA transcription factor TaIAA10-6D that bound to a conserved cis-regulatory module CCRM1-1, the most essential conserved cis-regulatory module in GLU-1. Here, we confirmed the binding of TaIAA10-6D to CCRM1-1 using yeast one hybrid and dual-luciferase reporter assays. The enhanced expression of TaIAA10-6D suppressed glutenin accumulation and increased gliadin content. Dynamic transcriptome analyses revealed that TaIAA10-6D overexpression down-regulated glutenin and gliadin genes during an early stage of grain filling, but up-regulated gliadin genes during a late stage probably by endoplasmic reticulum stress, accounting for its effect on the trade-off between glutenin and gliadin. Rheological property and processing quality assays showed that TaIAA10-6D overproduction reduced stabilization time and bread quality, but enhanced cookie quality. Overexpression of TaIAA10-6D also reduced plant height, leaf size, kernel number and grain yield. We identified two major haplotypes of TaIAA10-6D, Hap I and Hap II, and developed a breeding-friendly diagnostic marker. Hap I conferred higher expression of TaIAA10-6D and concomitantly reduced plant height and kernel number, but had little effect on grain yield, contributing to lodging resistance without yield penalty. Hap I was subjected to positive selection in breeding. The findings provide a useful gene for wheat improvement and broaden insights into the regulatory machinery underpinning auxin-mediated quality formation, plant morphogenesis and yield gain.
546			_aText in English
597			_dNational Natural Science Foundation of China _dChina Postdoctoral Science Foundation _dChinese Academy of Agricultural Sciences (CAAS)
650		7	_aAgronomic characters _2AGROVOC _91008
650		7	_aAuxins _2AGROVOC _91015
650		7	_aTranscription factors _2AGROVOC _96527
650		7	_aProcessing quality _2AGROVOC _99916
650		7	_aSeed storage proteins _2AGROVOC _912517
650		7	_aTriticum aestivum _2AGROVOC _91296
700	0		_aLina Xie _932205
700	0		_aHaibin Wu _940696
700	0		_aDengan Xu _95904
700	0		_aRui Che _940692
700	0		_aWenfei Tian _926337
700	0		_aBingyan Liu _932206
700	0		_aYuheng Chao _940691
700	0		_aYan Zhang _916460
700	0		_aXianchun Xia _9377
700	1		_aHe Zhonghu _gGlobal Wheat Program _8INT2411 _9838
700	0		_aShuanghe Cao _95093
773	0		_tCrop Journal _dChina : Elsevier B.V. ; KeAi Communications Co., 2025. _x2095-5421 _gv. 13, no. 5, p. 1460-1469 _w56924
856	4		_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/36188
942			_cJA _n0 _2ddc
999			_c69603 _d69595