000			02641nab a22003377a 4500
001			68946
003			MX-TxCIM
005			20250620155436.0
008			250609s2016 -uk|||p|op||| 00| 0 eng d
022			_a2046-2069 (Online)
024	8		_ahttps://doi.org/10.1039/C5RA20789D
040			_aMX-TxCIM
041			_aeng
100	0		_aZhanmin Liu _939296
245	1	0	_aColorimetric detection of Maize chlorotic mottle virus by reverse transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynapthol blue dye
260			_aUnited Kingdom : _bThe Royal Society of Chemistry, _c2016.
500			_aPeer review
520			_aMaize chlorotic mottle virus causes corn lethal necrosis disease, and can be transmitted via infected maize seeds. It remains a challenge to detect this virus to prevent its introduction, infection and wide transmission in fields. For this purpose, a colorimetric assay for the detection of Maize chlorotic mottle virus was developed, which utilizes RT-LAMP and hydroxynapthol blue dye (HNB). The reaction was performed for amplification in one step in a single tube at the optimum conditions (64 °C for 60 min, 150 μM HNB and 2 mM MgSO4). Samples infected with MCMV developed a characteristic sky blue color after the reaction but those uninfected with MCMV or infected with other plant pathogenic viruses did not. The results of the HNB staining method were reconfirmed through gel electrophoresis of the RT-LAMP products. The sensitivity of this assay was 4.8 pg μl−1 of RNA of Maize chlorotic mottle virus per reaction, which was approximately 10-fold higher sensitivity over a conventional RT-PCR test. The results indicate that this assay is highly species-specific, simple, low-cost, and visual for easy detection of Maize chlorotic mottle virus in plant tissues. Therefore, colorimetric detection of Maize chlorotic mottle virus is a potentially useful tool for middle or small-scale corporations and entry-exit inspection and quarantine bureaus to detect maize seeds or plant tissues infected with Maize chlorotic mottle virus.
546			_aText in English
650		7	_aMaize chlorotic mottle virus _2AGROVOC _99361
650		7	_aIsothermal processes _2AGROVOC _918087
650		7	_aGel electrophoresis _2AGROVOC _922497
650		7	_aRNA _2AGROVOC _95052
650		7	_aPlant tissues _2AGROVOC _913518
650		0	_aInfection _gAGROVOC _911032
700	0		_aXueying Xia _939297
700	0		_aCuiyun Yang _939298
700	0		_aJunyi Huang _939300
773	0		_dUnited Kingdom : The Royal Society of Chemistry, 2016. _gv. 6, p. 73–78 _tRSC Advances _x2046-2069
942			_2ddc _cJA _n0
999			_c68946 _d68938