000 03373nab|a22005297a|4500
001 68686
003 MX-TxCIM
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022 _a2045-2322 (Online)
024 8 _ahttps://doi.org/10.1038/s41598-025-91199-2
040 _aMX-TxCIM
041 _aeng
100 1 _aBashir, L.
_938507
245 1 0 _aIdentification of quantitative trait nucleotides for grain quality in bread wheat under heat stress
260 _aLondon (United Kingdom) :
_bNature Publishing Group,
_c2025.
500 _aPeer review
500 _aOpen Access
520 _aHeat stress is a critical factor affecting global wheat production and productivity. In this study, out of 500 studied germplasm lines, a diverse panel of 126 wheat genotypes grown under twelve distinct environmental conditions was analyzed. Using 35 K single-nucleotide polymorphism (SNP) genotyping assays and trait data on five biochemical parameters, including grain protein content (GPC), grain amylose content (GAC), grain total soluble sugars (TSS), grain iron (Fe), and zinc (Zn) content, six multi-locus GWAS (ML-GWAS) models were employed for association analysis. This revealed 67 stable quantitative trait nucleotides (QTNs) linked to grain quality parameters, explaining phenotypic variations ranging from 3 to 44.5% under heat stress conditions. By considering the results in consensus to at least three GWAS models and three locations, the final QTNs were reduced to 16, with 12 being novel findings. Notably, two novel markers, AX-94461119 (chromosome 2A) and AX-95220192 (chromosome 7D), associated with grain Fe and Zn, respectively, were validated through Kompetitive Allele Specific Polymerase Chain Reaction (KASP) approach. Candidate genes, including the P-loop-containing nucleoside triphosphate hydrolases (NTPases), Bowman-Birk type proteinase inhibitors (BBI), and the NPSN13 protein, were identified within associated genomic regions. These genes could serve as potential targets for enhancing quality traits and heat tolerance in future wheat improvement programs.
546 _aText in English
597 _dIndian Council of Agricultural Research (ICAR)
_fBreeding for Tomorrow
_uhttps://hdl.handle.net/10568/179132
650 7 _aHeat stress
_2AGROVOC
_91971
650 7 _aWheat
_2AGROVOC
_91310
650 7 _aGenome-wide association studies
_2AGROVOC
_931443
650 7 _aGrain
_2AGROVOC
_91138
650 7 _aQuality
_2AGROVOC
_91231
700 0 _aNeeraj Budhlakoti
_98360
700 1 _aPradhan, A.K.
_916709
700 1 _aSharma, D.
_938508
700 1 _aJain, A.
_938509
700 0 _aSaman Saim Rehman
_938510
700 1 _aKondal, V.
_938511
700 1 _aJacob, S.R.
_929516
700 1 _aBhardwaj, R.
_938512
700 1 _aGaikwad, K.
_938513
700 0 _aDwijesh Chandra Mishra
_98361
700 1 _aPandey. A.
_938514
700 1 _aKaur, S.
_924391
700 1 _aBhati, P. K.
_8001710151
_gBorlaug Institute for South Asia
_926310
700 1 _aSingh, R.
_925028
700 1 _aSingh, G.P.
_918282
700 1 _aKumar, S.
_92207
773 0 _tScientific Reports
_gv. 15, no. 1, art. 6641
_dLondon (United Kingdom) : Nature Publishing Group, 2025.
_x2045-2322
_wa58025
856 4 _yOpen Access through DSpace
_uhttps://hdl.handle.net/10883/35539
942 _cJA
_n0
_2ddc
999 _c68686
_d68678