| 000 | 02495nab a22004457a 4500 | ||
|---|---|---|---|
| 001 | 68072 | ||
| 003 | MX-TxCIM | ||
| 005 | 20250904111803.0 | ||
| 008 | 241106s1971 -us|||po p||| 00| 0 eng d | ||
| 022 | _a0021-8561 | ||
| 022 | _a1520-5118 (Online) | ||
| 024 | 8 | _ahttps://doi.org/10.1021/jf60178a011 | |
| 040 | _aMX-TxCIM | ||
| 041 | _aeng | ||
| 100 | 1 |
_aSodek, L. _937059 |
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| 245 | 1 | 0 | _aAmino acid compositions of proteins isolated from normal, opaque-2, and floury-2 corn endosperms by a modified Osborne procedure |
| 260 |
_aUnited States of America : _bAmerican Chemical Society, _c1971. |
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| 500 | _aPeer review | ||
| 520 | _aThe classical Osborne-Mendel extraction procedure for corn endosperm protein was modified to extract additional protein by a solvent containing 55% 2-propanol and 0.6% 2-mercaptoethanol. The new fraction is designated zein-2, since it has an amino acid composition somewhat similar to zein, but with higher levels of glycine, methionine, histidine, and proline, and lower levels of aspartic acid, leucine, and isoleucine. Zein-2 was previously recovered as a part of the glutelin fraction. Zein-1 and zein-2 , as well as glutelin, were heterogeneous by polyacrylamide gel electrophoresis. However. these three protein fractions had characteristic amino acid compositions which were constant for normal, opaque-2, and floury-2 endosperms. The two mutations which increase the lysine content of the endosperm act by changing the proportions of proteins which contain different levels of lysine. | ||
| 546 | _aText in English | ||
| 650 | 7 |
_aAmino acids _2AGROVOC _94464 |
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| 650 | 7 |
_aProtein content _2AGROVOC _91222 |
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| 650 | 7 |
_aMaize _2AGROVOC _91173 |
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| 650 | 7 |
_aEndosperm _2AGROVOC _91097 |
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| 650 | 7 |
_aSolvents _2AGROVOC _937060 |
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| 650 | 7 |
_aPropanol _2AGROVOC _937061 |
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| 650 | 7 |
_aZein _2AGROVOC _916128 |
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| 650 | 7 |
_aGlycine (amino acid) _2AGROVOC _918784 |
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| 650 | 7 |
_aMethionine _2AGROVOC _925829 |
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| 650 | 7 |
_aHistidine _2AGROVOC _937062 |
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| 650 | 7 |
_aProline _2AGROVOC _914165 |
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| 650 | 7 |
_aAspartic acid _2AGROVOC _936655 |
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| 650 | 7 |
_aLeucine _2AGROVOC _934136 |
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| 650 | 7 |
_aIsoleucine _2AGROVOC _933908 |
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| 650 | 7 |
_aGlutelins _2AGROVOC _934350 |
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| 650 | 7 |
_aPolyacrylamide gel electrophoresis _2AGROVOC _917755 |
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| 700 | 1 |
_aWilson, C.M. _934489 |
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| 773 | 0 |
_dUnited States of America : American Chemical Society, 1971. _gv. 19, no. 6, p. 1144–1150 _tJournal of Agricultural and Food Chemistry _w0021-8561 _xG444388 |
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| 942 |
_2ddc _cJA _n0 |
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| 999 |
_c68072 _d68064 |
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