000			03436nab|a22003977a|4500
001			67913
003			MX-TxCIM
005			20241031100212.0
008			20248s2024||||mx |||p|op||||00||0|eng|d
022			_a2076-0817 (Online)
024	8		_ahttps://doi.org/10.3390/pathogens13080656
040			_aMX-TxCIM
041			_aeng
100	0		_aMotasem Abumuslem _937001
245	1	0	_aMorphological and biochemical changes in the mediterranean cereal cyst nematode (Heterodera latipons) during diapause
260			_bMDPI, _c2024. _aSwitzerland :
500			_aPeer review
500			_aOpen Access
520			_aThe cereal cyst nematode (Heterodera latipons) is becoming an economically important species in global cereal production as it is being identified in many new cereal cultivated areas and causes significant losses. Consequently, understanding its biology becomes crucial for researchers in identifying its vulnerabilities and implementing effective control measures. In the current study, different morphological and biochemical changes of H. latipons cysts containing eggs with infective juveniles from a barley field in Jordan were studied during the summer of 2021, at two sample dates. The first, at the harvest of the cereal crop (June 2021), when the infective second-stage juveniles (J2s) were initiating diapause, and the second, before planting the sequent cereal crop (late October 2021), when the J2s were ending diapause. The studied population was characterized morphologically and molecularly, showing 98.4% molecular similarity to both JOD from Jordan and Syrian "300" isolates of H. latipons. The obtained results and observations revealed that there were dramatic changes in all the investigated features of the cysts and eggs they contained. Morphological changes such as cyst color, sub-crystalline layer, and thickness of the rigid eggshell wall were observed. A slight change in the emergence time of J2s from cysts was observed without any difference in the number of emerged J2s. The results of biochemical changes showed that the total contents of carbohydrates, glycogen, trehalose, glycerol, and protein were higher in cysts collected in October when compared to those cysts collected in June. The SDS-PAGE pattern indicated the presence of a protein with the size of ca. 100 kDa in both sampling dates, whereas another protein (ca. 20 kDa) was present only in the cysts of October. Furthermore, the expression of trehalase (tre) gene was detected only in H. latipons collected in October. The outcomes of this study provide new helpful information that elucidates diapause in H. latipons and may be used for the implementation of new management strategies of cyst nematodes.
546			_aText in English
650		7	_aEnzymes _2AGROVOC _94657
650		7	_aGenes _2AGROVOC _93563
650		7	_aMetabolism _2AGROVOC _910493
650		7	_aMolecular characterization _2AGROVOC _929016
650		7	_aMorphometrics _2AGROVOC _933057
650		7	_aTrehalase _2AGROVOC _937004
700	1		_aToktay, H. _91795
700	1		_aSadder, M.T. _937005
700	1		_aDababat, A.A. _gGlobal Wheat Program _8INT2918 _9874
700	0		_aNida’ M. Salem _937006
700	0		_aLuma Al-Banna _93199
773	0		_tPathogens _gv. 13, no. 8, art. 656 _dSwitzerland : MDPI, 2024. _x2076-0817
856	4		_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/34995
942			_cJA _n0 _2ddc
999			_c67913 _d67905