000			02081nab a22003737a 4500
001			67853
003			MX-TxCIM
005			20250311130900.0
008			240828s1978 -us|||p|op||| 00| 0 eng d
022			_a0021-8561
022			_a1520-5118 (Online)
024	8		_ahttps://doi.org/10.1021/jf60218a027
040			_aMX-TxCIM
041			_aeng
100	1		_aHagerman, A.E. _936678
245	1	0	_aProtein precipitation method for the quantitative determination of tannins
260			_aUnited States of America : _bAmerican Chemical Society, _c1978.
500			_aPeer review
520			_aThe tannin content of crude plant extracts or of purified preparations was determined by adding the sample to a standard solution of protein, isolating the insoluble tannin- protein complex, dissolving it in alkaline solution, and measuring the absorbance at 510 nm after adding ferric chloride. Plots of absorbance as a function of the amount of tannin are linear for tannic acid and partially purified sorghum tannins for amounts of tannin ranging from 0.20 to 1.0 mg. Nontannin components of crude methanolic extracts of sorghum and cowpeas do not interfere with the assay. The results of the precipitation method are qualitatively similar to results obtained with the vanillin assay. The precipitation assay can be used to study the effects of pH and other parameters on tannin- protein interactions.
546			_aText in English
650		7	_aProteins _2AGROVOC _91224
650		7	_aPrecipitation _2AGROVOC _99386
650		7	_aQuantitative analysis _2AGROVOC _95912
650		7	_aTannins _2AGROVOC _931904
650		7	_aIron compounds _2AGROVOC _936679
650		7	_aChlorides _2AGROVOC _95761
650		7	_aSorghum _2AGROVOC _92002
650		7	_aCowpeas _2AGROVOC _95144
650		7	_aVanillin _2AGROVOC _931903
650		7	_aPH effects _2AGROVOC _929991
700	1		_aButler, L.G. _931906
773	0		_dUnited States of America : American Chemical Society, 1978. _gv. 26, no. 4, p. 809–812 _tJournal of Agricultural and Food Chemistry _wG444388 _x0021-8561
942			_2ddc _cJA _n0
999			_c67853 _d67845