000 02561nab a22003857a 4500
001 67763
003 MX-TxCIM
005 20241217170134.0
008 240725s1987 -uk|||p|op||| 00| 0 eng d
022 _a1060-3271
022 _a1944-7922 (Online)
024 8 _ahttps://doi.org/10.1093/jaoac/70.1.171
040 _aMX-TxCIM
041 _aeng
100 1 _aGehrke, C.W.
_936580
245 1 0 _aQuantitative analysis of cystine, methionine, lysine, and nine other amino acids by a single oxidation-4 hour hydrolysis method
260 _aUnited Kingdom :
_bOxford University Press,
_c1987.
500 _aPeer review
520 _aThe sulfur-containing amino acids cystine and methionine play important roles in animal, especially avian, nutrition. Because these sulfur-containing amino acids are destroyed to varying extents by 6N HC1 hydrolysis, oxidation and hydrolysis of cystine to cysteic add and methionine to methionine sulfone have been widely used for determination of cystine and methionine. Lysine is considered the next limiting amino acid after the sulfur amino acids in poultry nutrition; therefore, determination of the amino acid content of rations focuses first on these 3 amino acids. The objective of this investigation was to establish whether lysine and other amino acids could be accurately determined in proteinaceous materials which had undergone performic acid oxidation. To perform this evaluation, lysine was determined in a variety of protein-containing materials both with and without performic acid oxidation. Performic acid oxidation followed by 6N HC1 hydrolysis at 145°C for 4 h allows accurate measurement of 3 amino acids especially important to poultry nutrition, cystine, methionine, and lysine, in a single preoxidized hydrolysate; this method can be extended to another 9 protein amino adds.
546 _aText in English
650 7 _aQuantitative analysis
_2AGROVOC
_95912
650 7 _aAmino acids
_2AGROVOC
_94464
650 7 _aCystine
_2AGROVOC
_934636
650 7 _aMethionine
_2AGROVOC
_925829
650 7 _aLysine
_2AGROVOC
_95257
650 7 _aOxidation
_2AGROVOC
_911786
650 7 _aHydrolysis
_2AGROVOC
_912240
650 7 _aSulfur amino acids
_2AGROVOC
_934638
700 1 _aRexroad, P.R.
_936581
700 1 _aSchisla, R.M.
_936582
700 1 _aAbsheer, J.S.
_936583
700 1 _aZumwalt, R.W.
_936584
773 0 _dUnited Kingdom : Oxford University Press, 1987.
_gv. 70, no. 1, p. 171–174
_tJournal of the Association of Official Analytical Chemists
_wG444584
_x1060-3271
942 _2ddc
_cJA
_n0
999 _c67763
_d67755