000			02628nab a22003497a 4500
001			67727
003			MX-TxCIM
005			20250923171258.0
008			240717s1984 gw |||p|op||| 00| 0 eng d
022			_a0022-2844
022			_a1432-1432 (Online)
024	8		_ahttps://doi.org/10.1007/BF02104739
040			_aMX-TxCIM
041			_aeng
100	1		_aWilson, D.R. _936492
245	1	0	_aZein gene organization in maize and related grasses
260			_aGermany : _bSpringer Nature, _c1984.
500			_aPeer review
520			_aZein cDNA clones were used to study the organization of zein genes within the genome of the inbred maize W64A. When individual clones for the two larger molecular-weight classes of zein proteins (Mr=22,000; Mr=19,000) were used as probes for Southern blot hybridizations of genomic DNA, multiple restriction fragments were found to hybridize. Reconstruction analyses using moderately stringent criteria were used to estimate a total of 70–80 zein sequences within the genome of this inbred maize. The hybridization patterns suggest that zein sequences are clustered within the same restriction fragment. When criteria permitting less cross-hybridization of homologous sequences (Tm-10°C) were used, the banding pattern changed, with some of the bands being reduced in intensity or eliminated entirely. Therefore, by control of hybridization criteria, particular zein genes may be more readily distinguished in a Southern blot analysis. The Southern blot hybridization pattern for the Mr=15,000 zein was less complex. Only a single major band was found, with sufficient hybridization intensity for two or three genes. Genomic Southern analyses of other inbred maizes and related grasses showed similarly complex hybridization patterns with cDNA probes for the 19,000- and 22,000-molecular-weight zeins, suggesting that these sequences have been conserved over evolutionary time. The zein multigene family may therefore have arisen by gene duplication before divergence of the maize, teosinte, and Tripsacum species from a common ancestor.
546			_aText in English
650		7	_aZein _2AGROVOC _916128
650		7	_aMultigene family _2AGROVOC _934462
650		7	_aComplementary DNA _2AGROVOC _934463
650		7	_aInbred lines _2AGROVOC _91155
650		0	_aHybridization _gAGROVOC _938416
650		7	_aMaize _2AGROVOC _91173
650		7	_aZea mexicana _2AGROVOC _99088
650		7	_aTripsacum _2AGROVOC _99988
700	1		_aLarkins, B.A. _9565
773	0		_dGermany : Springer Nature, 1984. _gv. 20, p. 330–340 _tJournal of Molecular Evolution _x0022-2844
942			_2ddc _cJA _n0
999			_c67727 _d67719