000			03347nab|a22004697a|4500
001			66721
003			MX-TxCIM
005			20240123224047.0
008			202312s2023||||mx |||p|op||||00||0|eng|d
022			_a0981-9428
022			_a1873-2690 (Online)
024	8		_ahttps://doi.org/10.1016/j.plaphy.2023.108188
040			_aMX-TxCIM
041			_aeng
100	0		_aWenqi Feng _928253
245	1	0	_aMaize ZmBES1/BZR1-1 transcription factor negatively regulates drought tolerance
260			_bElsevier, _c2023. _aFrance :
500			_aPeer review
520			_aDrought stress is a common abiotic factor and restricts plant growth and development. Exploring maize stress-related genes and their regulatory mechanisms is crucial for ensuring agricultural productivity and food security. The BRI1-EMS1 suppressor (BES1)/brassinazole-resistant 1 (BZR1) transcription factors (TFs) play important roles in plant growth, development, and stress response. However, maize ZmBES1/BZR1s are rarely reported. In the present study, the ZmBES1/BZR1-1 gene was cloned from maize B73 and functionally characterized in transgenic Arabidopsis and rice in drought stress response. The ZmBES1/BZR1-1 protein possessed a conserved bHLH domain characterized by BES1/BZR1 TFs, localized in the nucleus, and showed transcription activation activity. The expression of ZmBES1/BZR1-1 exhibited no tissue specificity but drought-inhibitory expression in maize. Under drought stress, overexpression of ZmBES1/BZR1-1 resulted in the enhancement of drought sensitivity of transgenic Arabidopsis and rice with a lower survival rate, reactive oxygen species (ROS) level and relative water content (RWC) and a higher stomatal aperture and relative electrolyte leakage (REL). The RNA-seq results showed that 56 differentially expressed genes (DEGs) were regulated by ZmBES1/BZR1-1 by binding to E-box elements in their promoters. The GO analysis showed that the DEGs were significantly annotated with response to oxidative stress and oxygen level. The study suggests that the ZmBES1/BZR1-1 gene negatively regulates drought stress, which provides insights into further underlying molecular mechanisms in the drought stress response mediated by BZR1/BES1s.
546			_aText in English
650		7	_aAbiotic stress _2AGROVOC _93448
650		7	_aDrought stress _2AGROVOC _91081
650		7	_aMaize _2AGROVOC _91173
650		7	_aTranscription factors _2AGROVOC _96527
700	0		_aHongwanjun Zhang _928255
700	0		_aYang Cao _932343
700	0		_aYuan Liu _913759
700	0		_aYiran Zhao _928254
700	0		_aFuai Sun _928256
700	0		_aQingqing Yang _928257
700	0		_aXuecai Zhang _8INT3400 _9951 _gGlobal Maize Program
700	0		_aYuanyuan Zhang _932344
700	0		_aYingge Wang _932345
700	0		_aWanchen Li _924648
700	0		_aYanli Lu _96408
700	0		_aFengling Fu _928258
700	0		_aHaoqiang Yu _928259
773	0		_tPlant Physiology and Biochemistry _gv. 205, art. 108188 _dFrance : Elsevier Masson s.r.l., 2023. _x0981-9428 _wu91708
942			_cJA _n0 _2ddc
999			_c66721 _d66713