000			02944nab|a22003737a|4500
001			66434
003			MX-TxCIM
005			20230906175907.0
008			20115s2011||||mx |||p|op||||00||0|eng|d
022			_a0022-1503
022			_a1465-7333 (Online)
024	8		_ahttps://doi.org/10.1093/jhered/esr015
040			_aMX-TxCIM
041			_aeng
100	1		_aAlo, F. _931586
245	1	0	_aLeveraging genomic resources of model species for the assessment of diversity and phylogeny in wild and domesticated lentil
260			_c2011. _aUnited Kingdom : _bOxford University Press,
500			_aPeer review
500			_aFree Access
520			_aAdvances in comparative genomics have provided significant opportunities for analysis of genetic diversity in species with limited genomic resources, such as the genus Lens. Medicago truncatula expressed sequence tags (ESTs) were aligned with the Arabidopsis thaliana genome sequence to identify conserved exon sequences and splice sites in the ESTs. Conserved primers (CPs) based on M. truncatula EST sequences flanking one or more introns were then designed. A total of 22% of the CPs produced polymerase chain reaction amplicons in lentil and were used to sequence amplicons in 175 wild and 133 domesticated lentil accessions. Analysis of the sequences confirmed that L. nigricans and L. ervoides are well-defined species at the DNA sequence level. Lens culinaris subsp. odemensis, L. culinaris subsp. tomentosus, and L. lamottei may constitute a single taxon pending verification with crossability experiments. Lens culinaris subsp. orientalis is the progenitor of domesticated lentil, L. culinaris subsp. culinaris (as proposed before), but a more specific area of origin can be suggested in southern Turkey. We were also able to detect the divergence, following domestication, of the domesticated gene pool into overlapping large-seeded (megasperma) and small-seeded (microsperma) groups. Lentil domestication led to a loss of genetic diversity of approximately 40%. The approach followed in this research has allowed us to rapidly exploit sequence information from model plant species for the study of genetic diversity of a crop such as lentil with limited genomic resources. © 2011 The American Genetic Association. All rights reserved.
546			_aText in English
591			_aFurman, B.J. : Not in IRS staff list but CIMMYT Affiliation
650		7	_aDomestication _2AGROVOC _930583
650		7	_aLens _2AGROVOC _931587
650		7	_aPhylogeny _2AGROVOC _99129
650		7	_aSingle nucleotide polymorphisms _2AGROVOC _910805
650		7	_aGenetic diversity (as resource) _2AGROVOC _92974
700	1		_aFurman, B.J. _931588
700	1		_aAkhunov, E. _93077
700	1		_aDvorak, J. _916465
700	1		_aGepts, P. _99157
773	0		_tJournal of Heredity _gv. 102, no. 3, p. 315-329 _dUnited Kingdom : Oxford University Press, 2011 _x0022-1503 _wG444556
942			_cJA _n0 _2ddc
999			_c66434 _d66426