000			02885nab|a22004097a|4500
001			66253
003			MX-TxCIM
005			20230428160110.0
008			20234s2023||||mx |||p|op||||00||0|eng|d
022			_a1471-2229
024	8		_ahttps://doi.org/10.1186/s12870-023-04188-w
040			_aMX-TxCIM
041			_aeng
100	0		_aRunmiao Tian _930758
245	1	0	_aMulti-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development
260			_bBioMed Central, _c2023. _aLondon (United Kingdom) :
500			_aPeer review
500			_aOpen Access
520			_aBackground: Glycosylphosphatidylinositol (GPI) and GPI-anchored proteins (GAPs) are important for cell wall formation and reproductive development in Arabidopsis. However, monocot counterparts that function in kernel endosperm development have yet to be discovered. Here, we performed a multi-omic analysis to explore the function of GPI related genes on kernel development in maize. Results: In maize, 48 counterparts of human GPI synthesis and lipid remodeling genes were identified, in which null mutation of the glucosaminyl-phosphatidylinositol O-acyltransferase1 gene, ZmGWT1, caused a kernel mutant (named gwt1) with defects in the basal endosperm transport layer (BETL). We performed plasma membrane (PM) proteomics to characterize the potential GAPs involved in kernel development. In total, 4,981 proteins were successfully identified in 10-DAP gwt1 kernels of mutant and wild-type (WT), including 1,638 membrane-anchored proteins with different posttranslational modifications. Forty-seven of the 256 predicted GAPs were differentially accumulated between gwt1 and WT. Two predicted BETL-specific GAPs (Zm00001d018837 and Zm00001d049834), which kept similar abundance at general proteome but with significantly decreased abundance at membrane proteome in gwt1 were highlighted. Conclusions: Our results show the importance of GPI and GAPs for endosperm development and provide candidate genes for further investigation of the regulatory network in which ZmGWT1 participates.
546			_aText in English
650		7	_aMaize _2AGROVOC _91173
650		7	_aEndosperm _2AGROVOC _91097
650		7	_aMembranes _2AGROVOC _930759
650		7	_aProteomics _2AGROVOC _918275
650		7	_aTranscriptomics _2AGROVOC _930116
700	0		_aJianjun Jiang _930760
700	1		_aShirong Bo _930761
700	0		_aHui Zhang _929229
700	0		_aXuehai Zhang _919695
700	1		_aHearne, S. _8INT3287 _9912 _gGenetic Resources Program
700	0		_aJihua Tang _919697
700	0		_aDong Ding _930762
700	0		_aZhiyuan Fu _919693
773	0		_tBMC Plant Biology _gv. 23, no. 1, p. 191 _dLondon (United Kingdom) : BioMed Central, 2023 _wG79387 _x1471-2229
856	4		_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/22580
942			_cJA _n0 _2ddc
999			_c66253 _d66245