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003			MX-TxCIM
005			20230818155620.0
008			20225s2022||||mx |||p|op||||00||0|eng|d
022			_a1664-8021 (Online)
024	8		_ahttps://doi.org/10.3389/fgene.2022.859676
040			_aMX-TxCIM
041			_aeng
100	1		_aSaxesena, R.R. _929519
245	1	0	_aSNP discovery using BSR-Seq approach for spot blotch resistance in wheat (Triticum aestivum L.), an essential crop for food security
260			_bFrontiers, _c2022. _aSwitzerland :
500			_aPeer review
500			_aOpen Access
520			_aThe pathogenic fungus, Bipolaris sorokiniana, that causes spot blotch (SB) disease of wheat, is a major production constraint in the Eastern Gangetic Plains of South Asia and other warm, humid regions of the world. A recombinant inbred line population was developed and phenotyped at three SB-prone locations in India. The single nucleotide polymorphism (SNP) for SB resistance was identified using a bulked segregant RNA-Seq-based approach, referred to as “BSR-Seq.” Transcriptome sequencing of the resistant parent (YS#24), the susceptible parent (YS#58), and their resistant and susceptible bulks yielded a total of 429.67 million raw reads. The bulk frequency ratio (BFR) of SNPs between the resistant and susceptible bulks was estimated, and selection of SNPs linked to resistance was done using sixfold enrichments in the corresponding bulks (BFR >6). With additional filtering criteria, the number of transcripts was further reduced to 506 with 1055 putative polymorphic SNPs distributed on 21 chromosomes of wheat. Based on SNP enrichment on chromosomal loci, five transcripts were found to be associated with SB resistance. Among the five SB resistance-associated transcripts, four were distributed on the 5B chromosome with putative 52 SNPs, whereas one transcript with eight SNPs was present on chromosome 3B. The SNPs linked to the trait were exposed to a tetra-primer ARMS-PCR assay, and an SNP-based allele-specific marker was identified for SB resistance. The in silico study of these five transcripts showed homology with pathogenesis-related genes; the metabolic pathway also exhibits similar results, suggesting their role in the plant defense mechanism.
546			_aText in English
650		7	_aWheat _2AGROVOC _91310
650		7	_aFood security _2AGROVOC _91118
650		0	_aSingle nucleotide polymorphisms _gAGROVOC _910805
650		7	_aDisease resistance _2AGROVOC _91077
700	0		_aVinod Kumar Mishra _91734
700	0		_aRamesh Chand _91735
700	1		_aKumar, U. _gFormerly Borlaug Institute for South Asia (BISA) _8INT3331 _9921
700	1		_aChowdhury, A.K. _92698
700	1		_aBhati, J. _929520
700	0		_aNeeraj Budhlakoti _98360
700	1		_aJoshi, A.K. _9873 _8INT2917 _gGlobal Wheat Program
773	0		_tFrontiers in Genetics _gv. 13, art. 859676 _dSwitzerland : Frontiers, 2022. _x1664-8021 _w58093
856	4		_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/22370
942			_cJA _n0 _2ddc
999			_c65837 _d65829