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022 _a0304-4238
024 _2https://doi.org/10.1016/j.scienta.2021.110859
040 _aMX-TxCIM
041 _aeng
100 1 _aSood, S.
_921901
245 1 0 _aKASP markers validation for late blight, PCN and PVY resistance in a large germplasm collection of tetraploid potato (Solanum tuberosum L.)
260 _aNetherlands :
_bElsevier,
_c2022.
500 _aPeer review
520 _aMolecular markers are important and valuable tools in crop breeding programmes to help in the discovery and manipulation of important genes governing resistance reaction. In potato, several biotic stresses hamper quantity and quality of tuber production. Availability of molecular markers and high throughput screening method provide an excellent opportunity to practice marker assisted selection for potato improvement without waiting for phenotypic screening. In this study, we developed 20 KASP markers related to late blight, PVY and PCN resistance traits to facilitate the screening at earlier stages of potato breeding programmes. Out of these 20 markers, nine KASP assays were validated and associated with the phenotype data on respective traits for reliability in their wider application. The validation was done in a panel of 1447 potato germplasm accessions including varieties from diverse provenience and advanced breeding clones. Among traits, the best association found related to KASP markers for late blight, namely markers snp ST00106, snp ST00020 and snp ST00023. Overall, the allele calls were conclusive for all the nine markers, and the results demonstrate the usefulness of SNP KASP markers in high throughput cost effective marker assisted selection (MAS) for important biotic stress traits in potatoes.
546 _aText in English
591 _aBohar, R. : Not in IRS staff list but CIMMYT Affiliation
650 7 _aBiotic stress
_2AGROVOC
_97593
650 7 _aGermplasm
_2AGROVOC
_91136
650 0 _aSingle nucleotide polymorphisms
_gAGROVOC
_910805
650 7 _aSolanum tuberosum
_2AGROVOC
_911757
700 1 _aBhardwaj, V.
_928819
700 1 _aChourasia, K.N
_928820
700 1 _aKaur, R.P.
_928821
700 1 _aKumar, V.
_913878
700 1 _aKumar, R.
_9560
700 1 _aSundaresha, S.
_928822
700 1 _aBohar, R.
_926368
700 1 _aGarcia Oliveira, A.L.
_96873
_8001712333
_gExcellence in Breeding
700 1 _aSingh R.K.
_919954
700 1 _aKumar, M.
_94728
773 0 _tScientia Horticulturae
_dNetherlands : Elsevier, 2022.
_x0304-4238
_gv. 295, art. 110859
_wu91711
942 _cJA
_n0
_2ddc
999 _c65605
_d65597