000			03753nab|a22004937a|4500
001			65099
003			MX-TxCIM
005			20231009164120.0
008			20221s22022|||msz ||p|op||||00||0|eengdd
022			_a1664-462X
024	8		_ahttps://doi.org/10.3389/fpls.2022.840614
040			_aMX-TxCIM
041			_aeng
100	0		_aJingyang Tong _919596
245	1	0	_aHigh resolution genome wide association studies reveal rich genetic architectures of grain zinc and iron in common wheat (Triticum aestivum L.)
260			_bFrontiers, _c2022. _aSwitzerland :
500			_aPeer review
500			_aOpen Access
520			_aBiofortification is a sustainable strategy to alleviate micronutrient deficiency in humans. It is necessary to improve grain zinc (GZnC) and iron concentrations (GFeC) in wheat based on genetic knowledge. However, the precise dissection of the genetic architecture underlying GZnC and GFeC remains challenging. In this study, high-resolution genome-wide association studies were conducted for GZnC and GFeC by three different models using 166 wheat cultivars and 373,106 polymorphic markers from the wheat 660K and 90K single nucleotide polymorphism (SNP) arrays. Totally, 25 and 16 stable loci were detected for GZnC and GFeC, respectively. Among them, 17 loci for GZnC and 8 for GFeC are likely to be new quantitative trait locus/loci (QTL). Based on gene annotations and expression profiles, 28 promising candidate genes were identified for Zn/Fe uptake (8), transport (11), storage (3), and regulations (6). Of them, 11 genes were putative wheat orthologs of known Arabidopsis and rice genes related to Zn/Fe homeostasis. A brief model, such as genes related to Zn/Fe homeostasis from root uptake, xylem transport to the final seed storage was proposed in wheat. Kompetitive allele-specific PCR (KASP) markers were successfully developed for two major QTL of GZnC on chromosome arms 3AL and 7AL, respectively, which were independent of thousand kernel weight and plant height. The 3AL QTL was further validated in a bi-parental population under multi-environments. A wheat multidrug and toxic compound extrusion (MATE) transporter TraesCS3A01G499300, the ortholog of rice gene OsPEZ2, was identified as a potential candidate gene. This study has advanced our knowledge of the genetic basis underlying GZnC and GFeC in wheat and provides valuable markers and candidate genes for wheat biofortification.
546			_aText in English
650		7	_aBiofortification _2AGROVOC _91731
650		7	_aIron _2AGROVOC _93544
650		7	_aZinc _2AGROVOC _91315
650		7	_aWheat _2AGROVOC _91310
650		7	_aGenes _2AGROVOC _93563
700	0		_aCong Zhao _926839
700	0		_aMengjing Sun _919597
700	0		_aLuping Fu _95903
700	0		_aJie Song _919337
700	0		_aDan Liu _919678
700	0		_aYelun Zhang _91855
700	0		_aJianmin Zheng _926840
700	0		_aZongjun Pu _919742
700	0		_aLianzheng Liu _926841
700	1		_aAwais Rasheed _gGlobal Wheat Program _8I1706474 _91938
700	0		_aMing Li _914174
700	0		_aXianchun Xia _9377
700	1		_aHe Zhonghu _9838 _gGlobal Wheat Program _8INT2411
700	1		_aYuanfeng Hao _9919
773	0		_tFrontiers in Plant Science _dSwitzerland : Frontiers, 2022 _x1664-462X _gv. 13, art. 840614 _w56875
856			_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/22021
942			_cJA _n0 _2ddc
999			_c65099 _d65091