000			02722nab|a22003737a|4500
001			65082
003			MX-TxCIM
005			20231013232458.0
008			20222s2022||||mx |||p|op||||00||0|eng|d
022			_a2223-7747
024	8		_ahttps://doi.org/10.3390/plants11030238
040			_aMX-TxCIM
041			_aeng
100	1		_aLopez-Gonzalez, C. _97657
245	1	0	_aAnalysis of global gene expression in maize (Zea mays) vegetative and reproductive tissues that differ in accumulation of starch and sucrose
260			_bMDPI, _c2022. _aBasel (Switzerland) :
500			_aPeer review
500			_aOpen Access
520			_aCarbon allocation between vegetative and reproductive tissues impacts cereal grain production. Despite great agricultural importance, sink–source relationships have not been fully characterized at the early reproductive stages in maize. Here, we quantify the accumulation of non-structural carbohydrates and patterns of gene expression in the top internode of the stem and the female inflorescence of maize at the onset of grain filling (reproductive stage R1). Top internode stem and female inflorescence tissues of the Puma maize inbred line were collected at reproductive stage R1 (without pollination) and non-structural carbohydrates were quantified by spectrophotometry. The female inflorescence accumulated starch at higher levels than the top internode of the stem. Global mRNA transcript levels were then evaluated in both tissues by RNA sequencing. Gene expression analysis identified 491 genes differentially expressed between the female inflorescence and the top stem internode. Gene ontology classification of differentially expressed genes showed enrichment for sucrose synthesis, the light-dependent reactions of photosynthesis, and transmembrane transporters. Our results suggest that sugar transporters play a key role in sugar partitioning in the maize stem and reveal previously uncharacterized differences between the female inflorescence and the top internode of the stem at early reproductive stages.
546			_aText in English
650		7	_aMaize _91173 _2AGROVOC
650		7	_aZea mays _91314 _2AGROVOC
650		7	_aSucrose _911941 _2AGROVOC
650		7	_aMetabolism _2AGROVOC _910493
650		7	_aInflorescences _926801 _2AGROVOC
700	1		_aJuarez-Colunga, S. _97654
700	1		_aTrachsel, S. _9914 _8INT3300 _gGlobal Maize Program
700	1		_aMarsch-Martínez, N. _926802
700	1		_aStewart Gillmor, C. _926803
700	1		_aTiessen, A. _97660
773	0		_tPlants _gv. 11, no. 3, art. 238 _dBasel (Switzerland) : MDPI, 2022 _x2223-7747
856			_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/22015
942			_cJA _n0 _2ddc
999			_c65082 _d65074