000			03634nab|a22004097a|4500
001			65075
003			MX-TxCIM
005			20231017232121.0
008			20221s2022||||mx |||p|op||||00||0|eng|d
022			_a2090-1232
024	8		_ahttps://doi.org/10.1016/j.jare.2022.01.010
040			_aMX-TxCIM
041			_aeng
100	1		_aBandyopadhyay, T. _926699
245	1		_aGWAS identifies genetic loci underlying nitrogen responsiveness in the climate resilient C4 model Setaria italica (L.)
260			_bElsevier, _c2022. _aEgypt :
500			_aPeer review
500			_aOpen Access
520			_aIntroduction: N responsiveness is the capacity to perceive and induce morpho-physiological adaptation to external and internal Nitrogen (N). Crop productivity is propelled by N fertilizer and requires the breeding/selection of cultivars with intrinsically high N responsiveness. This trait has many advantages in being more meaningful in commercial/environmental context, facilitating in-season N management and not being inversely correlated with N availability over processes regulating NUE. Current lack of its understanding at the physio-genetic basis is an impediment to select for cultivars with a predictably high N response. Objectives: To dissect physio-genetic basis of N responsiveness in 142 diverse population of foxtail millet, Setaria italica (L.) by employing contrasting N fertilizer nutrition regimes. Methods: We phenotyped S. italica accessions for major yield related traits under low (N10, N25) and optimal (N100) growth conditions and genotyped them to subsequently perform a genome-wide association study to identify genetic loci associated with nitrogen responsiveness trait. Groups of accessions showing contrasting trait performance and allelic forms of specific linked genetic loci (showing haplotypes) were further accessed for N dependent transcript abundances of their proximal genes. Results: Our study show that N dependent yield rise in S. italica is driven by grain number whose responsiveness to N availability is genetically underlined. We identify 22 unique SNP loci strongly associated with this trait out of which six exhibit haplotypes and consistent allelic variation between lines with contrasting N dependent grain number response and panicle architectures. Furthermore, differential transcript abundances of specific genes proximally linked to these SNPs in same lines is indicative of their N dependence in a genotype specific manner. Conclusion: The study demonstrates the value/ potential of N responsiveness as a selection trait and identifies key genetic components underlying the trait in S. italica. This has major implications for improving crop N sustainability and food security.
546			_aText in English
650		7	_aClimate _2AGROVOC _91558
650		7	_aResilience _2AGROVOC _95030
650		7	_aFood security _2AGROVOC _91118
650		7	_aGene Expression _2AGROVOC _98835
650		7	_aNitrogen _2AGROVOC _92912
700	1		_aSwarbreck, S.M. _925936
700	1		_aJaiswal, V. _926700
700	1		_aMaurya, J. _926701
700	1		_aGupta, R. _915173
700	1		_aBentley, A.R. _8001712492 _gFormerly Global Wheat Program _99599
700	1		_aGriffiths, H. _91922
700	1		_aPrasad, M. _926702
773	0		_tJournal of Advanced Research _dEgypt : Elsevier, 2022. _x2090-1232 _gv. 42, p. 249-261
856			_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/22010
942			_cJA _n0 _2ddc
999			_c65075 _d65067