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001			64954
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022			_a1664-462X (Online)
024	8		_ahttps://doi.org/10.3389/fpls.2021.782960
040			_aMX-TxCIM
041			_aeng
100	1		_8001712108 _aBiswal, A.K. _gFormerly Genetic Resources Program _918209
245	1		_aNovel mutant alleles reveal a role of the extra-large G protein in rice grain filling, panicle architecture, plant growth, and disease resistance
260			_bFrontiers, _c2022. _aSwitzerland :
500			_aPeer review
500			_aOpen Access
520			_aPlant growth and grain filling are the key agronomical traits for grain weight and yield of rice. The continuous improvement in rice yield is required for a future sustainable global economy and food security. The heterotrimeric G protein complex containing a canonical α subunit (RGA1) couples extracellular signals perceived by receptors to modulate cell function including plant development and grain weight. We hypothesized that, besides RGA1, three atypical, extra-large GTP-binding protein (XLG) subunits also regulate panicle architecture, plant growth, development, grain weight, and disease resistance. Here, we identified a role of XLGs in agronomic traits and stress tolerance by genetically ablating all three rice XLGs individually and in combination using the CRISPR/Cas9 genome editing in rice. For this study, eight (three single, two double, and three triple) null mutants were selected. Three XLG proteins combinatorically regulate seed filling, because loss confers a decrease in grain weight from 14% with loss of one XLG and loss of three to 32% decrease in grain weight. Null mutations in XLG2 and XLG4 increase grain size. The mutants showed significantly reduced panicle length and number per plant including lesser number of grains per panicle compared to the controls. Loss-of-function of all individual XLGs contributed to 9% more aerial biomass compared to wild type (WT). The double mutant showed improved salinity tolerance. Moreover, loss of the XLG gene family confers hypersensitivity to pathogens. Our findings suggest that the non-canonical XLGs play important roles in regulating rice plant growth, grain filling, panicle phenotype, stress tolerance, and disease resistance. Genetic manipulation of XLGs has the potential to improve agronomic properties in rice.
546			_aText in English
650		7	_aCRISPR _2AGROVOC _926508
650		7	_aRice _2AGROVOC _91243
650		7	_aProteins _2AGROVOC _91224
650		7	_aPlant growth _2AGROVOC _921209
650		7	_aDisease resistance _2AGROVOC _91077
700			_aWu, T.-Y.
700			_aUrano, D.
700			_aPelissier, R.
700			_aMorel, J.-B.
700			_aJones, A.M.
700			_aBiswal, A.K.
773	0		_tFrontiers in Plant Science _gv. 12, art. 782960 _dSwitzerland : Frontiers, 2022 _w56875 _x1664-462X
856			_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/21911
942			_cJA _n0 _2ddc
999			_c64954 _d64946