000			02877nab|a22003857a|4500
001			64899
003			MX-TxCIM
005			20220118194617.0
008			202109s2021||||xxu|||p|op||||00||0|eng|d
022			_a0031-949X
022			_a1943-7684 (Online)
024	8		_ahttps://doi.org/10.1094/PHYTO-11-20-0488-R
040			_aMX-TxCIM
041			_aeng
100	0		_aYuhui Zhang _917300
245	1	0	_aBasal rachis internode injection : _ba novel inoculation method to evaluate wheat resistance to Fusarium head blight
260			_aSt. Paul, MN (USA) : _bAmerican Phytopathological Society, _c2021.
500			_aPeer review
520			_aFusarium head blight (FHB) is one of the most destructive fungal diseases of wheat. However, difficulties in reliably phenotyping of this disease have greatly hindered the understanding of the mechanism of wheat-pathogen interaction and genetic improvement of FHB resistance. Here we report a novel inoculation method called basal rachis internode injection (BRII), in which inoculum is injected into the basal internode of a rachis instead of a floret, as is done in single floret inoculation (SFI). One of the prominent advantages of BRII over SFI and other traditional methods lies in its independence from the moisture-maintaining system that is necessary for all existing methods, making it insensitive to environmental humidity and hence cost-effective. Another unique feature of BRII is that this method produces nearly clear-cut reaction types, by which FHB resistance can be treated as a qualitative trait because generally no FHB symptoms appear on the spikelets of resistant genotypes. In addition, BRII outperformed SFI with a higher infection rate and better goodness of fit with known FHB resistance and quantitative trait locus components in a panel of 15 genotypes, as well as two populations of recombinant inbred lines segregating in Fhb1. Note that BRII and SFI methods are not mutually exclusive but rather complementary because each method has its own advantages in differentiating FHB resistance between genotypes. Combining these two methods would significantly improve the reliability and consistency of FHB phenotyping in wheat.
546			_aText in English
650		7	_aDisease resistance _91077 _2AGROVOC
650		7	_aFusarium _92705 _2AGROVOC
650		7	_aHost parasite relations _99585 _2AGROVOC
650		7	_aPhenotypes _93634 _2AGROVOC
650		7	_aTechniques _913203 _2AGROVOC
650		7	_aWheat _91310 _2AGROVOC
700	0		_aAiai Li _926462
700	0		_aSuqin Zhu _926463
700	0		_aLei Li _917301
700	1		_aXinyao He _gGlobal Wheat Program _8INT3297 _9913
700	0		_aZhengxi Sun _917302
700	0		_aTao Li _93707
773	0		_tPhytopathology _gv. 111, no. 9, p. 1670-1674 _dSt. Paul, MN (USA) : American Phytopathological Society, 2021 _x0031-949X _wG444676
942			_cJA _n0 _2ddc
999			_c64899 _d64891