| 000 | 02418nab|a22003377a|4500 | ||
|---|---|---|---|
| 001 | 64745 | ||
| 003 | MX-TxCIM | ||
| 005 | 20211217230540.0 | ||
| 008 | 200712s2015||||xxu|||p|op||||00||0|eng|d | ||
| 022 | _a0031-949X | ||
| 022 | _a1943-7684 (Online) | ||
| 024 | 8 | _ahttps://doi.org/10.1094/PHYTO-09-14-0250-R | |
| 040 | _aMX-TxCIM | ||
| 041 | _aeng | ||
| 100 | 1 |
_aEdwards, M.C. _926127 |
|
| 245 | 1 | 0 | _aInfectious Maize rayado fino virus from Cloned cDNA |
| 260 |
_aSt. Paul, MN (USA) : _bAmerican Phytopathological Society, _c2015. |
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| 500 | _aPeer review | ||
| 500 | _aOpen Access | ||
| 520 | _aA full-length cDNA clone was produced from a U.S. isolate of Maize rayado fino virus (MRFV), the type member of the genus Marafivirus within the family Tymoviridae. Infectivity of transcripts derived from cDNA clones was demonstrated by infection of maize plants and protoplasts, as well as by transmission via the known leafhopper vectors Dalbulus maidis and Graminella nigrifrons that transmit the virus in a persistent-propagative manner. Infection of maize plants through vascular puncture inoculation of seed with transcript RNA resulted in the induction of fine stipple stripe symptoms typical of those produced by wild-type MRFV and a frequency of infection comparable with that of the wild type. Northern and Western blotting confirmed the production of MRFV-specific RNAs and proteins in infected plants and protoplasts. An unanticipated increase in subgenomic RNA synthesis over levels in infected plants was observed in protoplasts infected with either wild-type or cloned virus. A conserved cleavage site motif previously demonstrated to function in both Oat blue dwarf virus capsid protein and tymoviral nonstructural protein processing was identified near the amino terminus of the MRFV replicase polyprotein, suggesting that cleavage at this site also may occur. | ||
| 546 | _aText in English | ||
| 650 | 7 |
_2AGROVOC _926128 _aMaize rayado fino virus |
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| 650 | 7 |
_2AGROVOC _926129 _acDNA libraries |
|
| 650 | 7 |
_2AGROVOC _916507 _aRNA viruses |
|
| 700 | 1 |
_926130 _aWeiland, J.J. |
|
| 700 | 1 |
_926131 _aTodd, J. |
|
| 700 | 1 |
_913717 _aStewart, L.R. |
|
| 773 | 0 |
_dSt. Paul, MN (USA) : American Phytopathological Society, 2015. _gv. 105, no. 6, p. 833-839 _tPhytopathology _w0031-949X _x444676 |
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| 856 | 4 |
_uhttps://doi.org/10.1094/PHYTO-09-14-0250-R _yClick here to access online |
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| 942 |
_cJA _n0 _2ddc |
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| 999 |
_c64745 _d64737 |
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