000			03148nab a22004217a 4500
001			64733
003			MX-TxCIM
005			20211217230332.0
008			200115s2019 sz |||p|op||| 00| 0 eng d
022			_a1422-0067 (Online)
024	8		_ahttps://doi.org/10.3390/ijms20112769
040			_aMX-TxCIM
041			_aeng
100	1		_926072 _aRevskij, D.
245	1	0	_aDietary fatty acids affect red blood cell membrane composition and red blood cell ATP release in dairy cows
260			_aBasel (Switzerland) : _bMDPI, _c2019.
500			_aPeer review
500			_aOpen Access
520			_aDiets of dairy cows are often based on maize silage (MS), delivering lower amounts of n-3 fatty acids (FA) compared to grass silage-based diets. The fatty acid composition of the cell membrane can affect the cell function. We evaluated the effects of an MS-based diet on bovine red blood cell (RBC) membrane FA composition and dietary effects on controlled ATP release of RBC. In trial 1, German Holstein cows were fed an MS-based total mixed ration for 24 weeks. The FA composition of RBC membranes from repeatedly taken blood samples was analysed in addition to the abundance of the RBC membrane protein flotillin-1, which is involved in, for example, cell signalling. In trial 2, four rumen fistulated MS-fed cows were abomasally infused in a 4 × 4 Latin square model with three successively increasing lipid dosages (coconut oil, linseed–safflower oil mix (EFA; rich in n-3 FA), Lutalin®, providing conjugated linoleic acids (CLA) or the combination of the supplements, EFA + CLA) for six weeks, followed by a three-week washout period. In trial 2, we analysed RBC ATP release, flotillin-1, and the membrane protein abundance of pannexin-1, which is involved in ATP release as the last part of a signalling cascade. In trial 1, the total amount of n-3 FA in RBC membranes decreased and the flotillin-1 abundance increased over time. In trial 2, the RBC n-3 FA amount was higher after the six-week infusion period of EFA or EFA + CLA. Furthermore, depending on the dosage of FA, the ATP release from RBC increased. The abundance of flotillin-1 and pannexin-1 was not affected in trial 2. It is concluded that changes of the membrane FA composition influence the RBC function, leading to altered ATP release from intact bovine RBC.
546			_aText in English
650		7	_2AGROVOC _913572 _aDairy cows
650		7	_2AGROVOC _926073 _aErythrocytes
650		7	_2AGROVOC _912373 _aFatty acids
650		7	_2AGROVOC _917818 _aATP
700	1		_926074 _aHaubold, S.
700	1		_926075 _aViergutz, T.
700	1		_926076 _aKröger-Koch, C.
700	1		_926077 _aTuchscherer, A.
700	1		_926078 _aKienberger, H.
700	1		_926079 _aRychlik, M.
700	1		_926080 _aTröscher, A.
700	1		_926081 _aHammon, H.M.
700	1		_926082 _aSchuberth, H.J.
700	1		_926083 _aMielenz, M.
773	0		_dBasel (Switzerland) : MDPI, 2019. _gv. 20, no. 11, art. 2769 _tInternational Journal of Molecular Sciences _w57216 _x1422-0067
856	4		_yClick here to access online _uhttps://doi.org/10.3390/ijms20112769
942			_2ddc _cJA _n0
999			_c64733 _d64725