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001			64731
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005			20211217230304.0
008			200115s2018 sz |||p|op||| 00| 0 eng d
022			_a1422-0067 (Online)
024	8		_ahttps://doi.org/10.3390/ijms19103225
040			_aMX-TxCIM
041			_aeng
100	1		_926056 _aZenda, T.
245	1	0	_aComparative proteomic and physiological analyses of two divergent maize inbred lines provide more insights into drought-stress tolerance mechanisms
260			_aBasel (Switzerland) : _bMDPI, _c2018.
500			_aPeer review
500			_aOpen Access
520			_aDrought stress is the major abiotic factor threatening maize (Zea mays L.) yield globally. Therefore, revealing the molecular mechanisms fundamental to drought tolerance in maize becomes imperative. Herein, we conducted a comprehensive comparative analysis of two maize inbred lines contrasting in drought stress tolerance based on their physiological and proteomic responses at the seedling stage. Our observations showed that divergent stress tolerance mechanisms exist between the two inbred-lines at physiological and proteomic levels, with YE8112 being comparatively more tolerant than MO17 owing to its maintenance of higher relative leaf water and proline contents, greater increase in peroxidase (POD) activity, along with decreased level of lipid peroxidation under stressed conditions. Using an iTRAQ (isobaric tags for relative and absolute quantification)-based method, we identified a total of 721 differentially abundant proteins (DAPs). Amongst these, we fished out five essential sets of drought responsive DAPs, including 13 DAPs specific to YE8112, 107 specific DAPs shared between drought-sensitive and drought-tolerant lines after drought treatment (SD_TD), three DAPs of YE8112 also regulated in SD_TD, 84 DAPs unique to MO17, and five overlapping DAPs between the two inbred lines. The most significantly enriched DAPs in YE8112 were associated with the photosynthesis antenna proteins pathway, whilst those in MO17 were related to C5-branched dibasic acid metabolism and RNA transport pathways. The changes in protein abundance were consistent with the observed physiological characterizations of the two inbred lines. Further, quantitative real-time polymerase chain reaction (qRT-PCR) analysis results confirmed the iTRAQ sequencing data. The higher drought tolerance of YE8112 was attributed to: activation of photosynthesis proteins involved in balancing light capture and utilization; enhanced lipid-metabolism; development of abiotic and biotic cross-tolerance mechanisms; increased cellular detoxification capacity; activation of chaperones that stabilize other proteins against drought-induced denaturation; and reduced synthesis of redundant proteins to help save energy to battle drought stress. These findings provide further insights into the molecular signatures underpinning maize drought stress tolerance.
546			_aText in English
650		7	_2AGROVOC _918275 _aProteomics
650		7	_2AGROVOC _91224 _aProteins
650		7	_2AGROVOC _91081 _aDrought stress
650		7	_2AGROVOC _926057 _aPhysiological response
650		7	_2AGROVOC _91314 _aZea mays
700	0		_926058 _aSongtao Liu
700	0		_926059 _aXuan Wang
700	0		_926060 _aHongyu Jin
700	0		_926061 _aGuo Liu
700	0		_926062 _aHuijun Duan
773	0		_dBasel (Switzerland) : MDPI, 2018. _gv. 19, no. 10, art. 3225 _tInternational Journal of Molecular Sciences _w57216 _x1422-0067
856	4		_yClick here to access online _uhttps://doi.org/10.3390/ijms19103225
942			_2ddc _cJA _n0
999			_c64731 _d64723