| 000 | 02247nab|a22003977a|4500 | ||
|---|---|---|---|
| 001 | 64681 | ||
| 003 | MX-TxCIM | ||
| 005 | 20211213160026.0 | ||
| 008 | 20211s2006||||xxk|||p|op||||00||0|eng|d | ||
| 022 | _a0736-6205 | ||
| 022 | _a1940-9818 (Online) | ||
| 024 | 8 | _ahttps://doi.org/10.2144/000112244 | |
| 040 | _aMX-TxCIM | ||
| 041 | _aeng | ||
| 100 | 0 |
_aShi-You Ding _925757 |
|
| 245 | 1 | 0 | _aVersatile derivatives of carbohydrate-binding modules for imaging of complex carbohydrates approaching the molecular level of resolution |
| 260 |
_aLondon (United Kingdom) : _bFuture Science, _c2006. |
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| 500 | _aPeer review | ||
| 500 | _aOpen Access | ||
| 520 | _aThe innate binding specificity of different carbohydrate-binding modules (CBMs) offers a versatile approach for mapping the chemistry and structure of surfaces that contain complex carbohydrates. We have employed the distinct recognition properties of a double His-tagged recombinant CBM tagged with semiconductor quantum dots for direct imaging of crystalline cellulose at the molecular level of resolution, using transmission and scanning transmission electron microscopy. In addition, three different types of CBMs from families 3, 6, and 20 that exhibit different carbohydrate specificities were each fused with either green fluorescent protein (GFP) or red fluorescent protein (RFP) and employed for double-labeling fluorescence microscopy studies of primary cell walls and various mixtures of complex carbohydrate target molecules. CBM probes can be used for characterizing both native complex carbohydrates and engineered biomaterials. | ||
| 546 | _aText in English | ||
| 650 | 7 |
_2AGROVOC _94730 _aCarbohydrates |
|
| 650 | 7 |
_2AGROVOC _910231 _aImagery |
|
| 700 | 0 |
_aQi Xu _925758 |
|
| 700 | 1 |
_aAli, M.K. _925759 |
|
| 700 | 1 |
_aBaker, J.O. _925760 |
|
| 700 | 1 |
_aBayer, E.A. _925761 |
|
| 700 | 1 |
_aBarak, Y. _925762 |
|
| 700 | 1 |
_aLamed, R. _925763 |
|
| 700 | 0 |
_aJunji Sugiyama _925764 |
|
| 700 | 1 |
_aRumbles, G. _925765 |
|
| 700 | 1 |
_aHimmel, M.E. _925766 |
|
| 773 | 0 |
_tBioTechniques _dLondon (United Kingdom) : Future Science, 2006. _x0736-6205 _gv. 41, no. 4, p. 435-443 |
|
| 856 | 4 |
_uhttps://doi.org/10.2144/000112244 _yClick here to access online |
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| 942 |
_cJA _n0 _2ddc |
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| 999 |
_c64681 _d64673 |
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