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003			MX-TxCIM
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008			202102s2020||||xxk|||p|op||||00||0|eng|d
022			_a2045-2322 (Online)
024	8		_ahttps://doi.org/10.1038/s41598-020-64249-0
040			_aMX-TxCIM
041			_aeng
100	1		_aMasters-Clark, E. _925479
245	1	0	_aDevelopment of a defined compost system for the study of plant-microbe interactions
260			_aLondon (United Kingdom) : _bNature Publishing Group, _c2020.
500			_aPeer review
500			_aOpen Access
520			_aPlant growth promoting rhizobacteria can improve plant health by providing enhanced nutrition, disease suppression and abiotic stress resistance, and have potential to contribute to sustainable agriculture. We have developed a sphagnum peat-based compost platform for investigating plant-microbe interactions. The chemical, physical and biological status of the system can be manipulated to understand the relative importance of these factors for plant health, demonstrated using three case studies: 1. Nutrient depleted compost retained its structure, but plants grown in this medium were severely stunted in growth due to removal of essential soluble nutrients - particularly, nitrogen, phosphorus and potassium. Compost nutrient status was replenished with the addition of selected soluble nutrients, validated by plant biomass; 2. When comparing milled and unmilled compost, we found nutrient status to be more important than matrix structure for plant growth; 3. In compost deficient in soluble P, supplemented with an insoluble inorganic form of P (Ca3(PO4)2), application of a phosphate solubilising Pseudomonas strain to plant roots provides a significant growth boost when compared with a Pseudomonas strain incapable of solubilising Ca3(PO4)2. Our findings show that the compost system can be manipulated to impose biotic and abiotic stresses for testing how microbial inoculants influence plant growth.
546			_aText in English
650		0	_aMicroorganisms _gAGROVOC _910080
650		7	_aRhizobacteria _2AGROVOC _915096
650		7	_aPlant growth _2AGROVOC _921209
650		7	_aSoil micro-organisms _2AGROVOC _925480
700	1		_aShone, E. _925481
700	1		_aParadelo, M. _925482
700	1		_aHirsch, P.R. _916483
700	1		_aClark, I. _916480
700	1		_aOtten, W. _925483
700	1		_aBrennan, F. _925484
700	1		_aMauchline, T.H. _916485
773	0		_gv. 10, art. 7521 _dLondon : Nature Publishing Group, 2020. _x2045-2322 _tNature Scientific Reports _wa58025
856	4		_yClick here to access online _uhttps://doi.org/10.1038/s41598-020-64249-0
942			_cJA _n0 _2ddc
999			_c64626 _d64618