000			03100nab|a22004577a|4500
999			_c64198 _d64190
001			64198
003			MX-TxCIM
005			20230731172211.0
008			202101s2020||||ne |||p|op||||00||0|eng|d
022			_a1380-3743
022			_a1572-9788 (Online)
024	8		_ahttps://doi.org/10.1007/s11032-020-01163-3
040			_aMX-TxCIM
041			_aeng
100	0		_aKunhui He _922961
245	1	0	_aMining of candidate genes for nitrogen use efficiency in maize based on genome-wide association study
260			_aDordrecht (Netherlands) : _bSpringer, _c2020.
500			_aPeer review
520			_aImproving the nitrogen use efficiency (NUE) may considerably increase maize yield and decrease the use of nitrogen (N) fertilizer. But the genetic basis of NUE in maize is still poorly understood. In this study, an association panel of 139 maize inbred lines genotyped with 50,790 single nucleotide polymorphism (SNP) was used to dissect the genetic basis of NUE-related traits by genome-wide association study (GWAS). NUE, N uptake efficiency (NupE), N utilization efficiency (NutE), grain N concentration (GNC), stover N concentration (SNC) and N harvest index (NHI) were estimated under two N levels. GWAS was performed using a fixed and random model circulating probability unification (FarmCUP) method. In total, 27 and 23 significant SNP-traits association signals were identified under normal and low N levels. In addition, 10 significant association signals were detected based on the traits relative value (normal N supply / low N supply) of two N levels. Further, 60 candidate genes were predicted for these traits base on linkage disequilibrium (LD) and low nitrogen transcriptome analysis of significant SNP regions. Among the candidate genes identified in this study, 66.7% involved in nitrogen compound metabolic process. Zm00001d025831 and Zm00001d004633 encoded ammonium transporter1 and transmembrane amino acid transporter family protein, respectively, may be important candidate genes for NUE. The markers identified in this study maybe has important significance and could be useful in molecular marker assisted selection in breeding of high-NUE maize varieties, and the candidate genes could deepen the understanding of the genetic basis of NUE.
546			_aText in English
650		7	_aMaize _gAGROVOC _2 _91173
650		7	_aNitrogen _2AGROVOC _92912
650		0	_aSingle nucleotide polymorphisms _gAGROVOC _910805
650		7	_aGenes _2AGROVOC _93563
700	0		_aShuTu Xu _919530
700	0		_aXinghua Zhang _922962
700	0		_aYanan Li _922963
700	0		_aLiguo Chang _922964
700	0		_aYahui Wang _922965
700	0		_aYaqin Shi _922966
700	0		_aTingting Cui _922967
700	0		_aYuan Dong _922968
700	0		_aTianru Lan _922969
700	0		_aXueyan Liu _918573
700	0		_aYutang Du _922970
700	0		_aRenhe Zhang _922971
700	0		_aJianchao Liu _922972
700	0		_aJiquan Xue _922973
773	0		_tMolecular Breeding _dDordrecht (Netherlands) : Springer, 2020. _x1380-3743 _gv. 40, no. 9, art. 83 _wu78961
942			_cJA _n0 _2ddc