000			03527nab|a22004337a|4500
999			_c64173 _d64165
001			64173
003			MX-TxCIM
005			20240919021231.0
008			201307s2013||||xxu|||p|op||||00||0|eng|d
022			_a1932-6203
024	8		_ahttps://doi.org/10.1371/journal.pone.0070041
040			_aMX-TxCIM
041			_aeng
100	1		_aMuchero, W. _922813
245	1	0	_aGenetic architecture of delayed senescence, biomass, and grain yield under drought stress in cowpea
260			_aSan Francisco, CA (USA) : _bPublic Library of Science, _c2013.
500			_aPeer review
500			_aOpen Access
520			_aThe stay-green phenomenon is a key plant trait with wide usage in managing crop production under limited water conditions. This trait enhances delayed senescence, biomass, and grain yield under drought stress. In this study we sought to identify QTLs in cowpea (Vigna unguiculata) consistent across experiments conducted in Burkina Faso, Nigeria, Senegal, and the United States of America under limited water conditions. A panel of 383 diverse cowpea accessions and a recombinant inbred line population (RIL) were SNP genotyped using an Illumina 1536 GoldenGate assay. Phenotypic data from thirteen experiments conducted across the four countries were used to identify SNP-trait associations based on linkage disequilibrium association mapping, with bi-parental QTL mapping as a complementary strategy. We identified seven loci, five of which exhibited evidence suggesting pleiotropic effects (stay-green) between delayed senescence, biomass, and grain yield. Further, we provide evidence suggesting the existence of positive pleiotropy in cowpea based on positively correlated mean phenotypic values (0.34< r <0.87) and allele effects (0.07< r <0.86) for delayed senescence and grain yield across three African environments. Three of the five putative stay-green QTLs, Dro-1, 3, and 7 were identified in both RILs and diverse germplasm with resolutions of 3.2 cM or less for each of the three loci, suggesting that these may be valuable targets for marker-assisted breeding in cowpea. Also, the co-location of early vegetative delayed senescence with biomass and grain yield QTLs suggests the possibility of using delayed senescence at the seedling stage as a rapid screening tool for post-flowering drought tolerance in cowpea breeding. BLAST analysis using EST sequences harboring SNPs with the highest associations provided a genomic context for loci identified in this study in closely related common bean (Phaseolus vulgaris) and soybean (Glycine max) reference genomes.
546			_aText in English
650		7	_aQuantitative Trait Loci _2AGROVOC _91853
650		0	_aSingle nucleotide polymorphisms _gAGROVOC _910805
650		7	_aChromosome mapping _2AGROVOC _92084
650		7	_aPlant Genetics _2AGROVOC _99025
650		7	_aDrought _2AGROVOC _91080
650		7	_aGenomics _2AGROVOC _91132
650		7	_aPlant breeding _gAGROVOC _2 _91203
700	1		_aRoberts, P.A. _922814
700	1		_aDiop, N.N. _8INT3187 _9897 _gGeneration Challenge Program
700	1		_aDrabo, I. _922815
700	1		_aCisse, N. _922816
700	1		_aClose, T.J. _917858
700	0		_aSatoru Muranaka _918109
700	1		_aBoukar, O. _915443
700	1		_aEhlers, J.D. _922817
773	0		_tPLoS ONE _gv. 8, no. 7, e70041 _dSan Francisco, CA (USA) : Public Library of Science, 2013. _wG94957 _x1932-6203
856	4		_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/21642
942			_cJA _n0 _2ddc