000			02620nab|a22004217a|4500
999			_c64136 _d64128
001			64136
003			MX-TxCIM
005			20211006085231.0
008			200910s2021||||xxk|||p|op||||00||0|eng|d
022			_a1756-0500
024	8		_ahttps://doi.org/10.1186/s13104-021-05483-1
040			_aMX-TxCIM
041			_aeng
100	1		_aDjankpa, F.T. _922496
245	1	0	_aAssessment of corn starch as substitute for agarose in DNA gel electrophoresis
260			_aLondon (United Kingdom) : _bBioMed Central, _c2021.
500			_aPeer review
500			_aOpen Access
520			_aObjective: The use of agarose in nucleic acid electrophoresis is the gold standard. However, agarose is very expensive and not readily available in resource limited developing countries like Ghana. Hence, finding a more affordable and readily available alternative to agarose will be a major boost to molecular research in developing countries. This study was aimed at investigating the use of corn starch as a potential substitute for agarose in DNA gel electrophoresis. Results: Genomic deoxyribonucleic acid (DNA) extracted from Plasmodium falciparum and primers were obtained from the West African Centre for Cell Biology of Infectious Pathogens and amplified using polymerase chain reaction. The amplicon was run on agarose gel to ascertain the molecular weight (as a positive control). When visualized under both blue light and ultraviolet light, the DNA and ladder showed clear and clean bands with the expected molecular weight. Corn starch was then modified with sodium borate buffer, casted into a gel and used to run the same DNA sample. Our findings indicated that similar to agarose, the DNA sample and ladder migrated successfully through the modified starch gel but no bands were visible when visualized under blue and ultra-violet light.
546			_aText in English
650		7	_2AGROVOC _922497 _aGel electrophoresis
650		7	_2AGROVOC _913434 _aDNA
650		7	_aMaize _gAGROVOC _2 _91173
650		7	_2AGROVOC _95810 _aStarch
650		7	_2AGROVOC _922498 _aAgarose
700	1		_922499 _aWiafe, G.A.
700	1		_922500 _aBoateng, B.N.
700	1		_922501 _aTsegah, K.M.
700	1		_922502 _aEssien-Baidoo, S.
700	1		_922503 _aUlanja, M.B.
700	1		_922504 _aAffram, K.O.
700	1		_922505 _aUssif, A.M.
700	1		_922506 _aAgyeman, D.O.
700	1		_922507 _aAsante, G.
773	0		_tBMC Research Notes _gv. 14, art. 73 _dLondon (United Kingdom) : BioMed Central, 2021. _x1756-0500
856	4		_yClick here to access online _uhttps://doi.org/10.1186/s13104-021-05483-1
942			_cJA _n0 _2ddc