000			03463nab a22004577a 4500
999			_c64131 _d64123
001			64131
003			MX-TxCIM
005			20211006074802.0
008			210625s2021 xxk|||p|op||| 00| 0 eng d
022			_a1754-6834
024	8		_ahttps://doi.org/10.1186/s13068-021-01927-9
040			_aMX-TxCIM
041			_aeng
100	1		_922463 _aBrandt, S.C.
245	1	0	_aInsights into the genome and secretome of Fusarium metavorans DSM105788 by cultivation on agro-residual biomass and synthetic nutrient sources
260			_aLondon (United Kingdom) : _bBioMed Central, _c2021.
500			_aPeer review
500			_aOpen Access
520			_aBackground: The transition to a biobased economy involving the depolymerization and fermentation of renewable agro-industrial sources is a challenge that can only be met by achieving the efficient hydrolysis of biomass to monosaccharides. In nature, lignocellulosic biomass is mainly decomposed by fungi. We recently identified six efficient cellulose degraders by screening fungi from Vietnam. Results: We characterized a high-performance cellulase-producing strain, with an activity of 0.06 U/mg, which was identified as a member of the Fusarium solani species complex linkage 6 (Fusarium metavorans), isolated from mangrove wood (FW16.1, deposited as DSM105788). The genome, representing nine potential chromosomes, was sequenced using PacBio and Illumina technology. In-depth secretome analysis using six different synthetic and artificial cellulose substrates and two agro-industrial waste products identified 500 proteins, including 135 enzymes assigned to five different carbohydrate-active enzyme (CAZyme) classes. The F. metavorans enzyme cocktail was tested for saccharification activity on pre-treated sugarcane bagasse, as well as untreated sugarcane bagasse and maize leaves, where it was complemented with the commercial enzyme mixture Accellerase 1500. In the untreated sugarcane bagasse and maize leaves, initial cell wall degradation was observed in the presence of at least 196 µg/mL of the in-house cocktail. Increasing the dose to 336 µg/mL facilitated the saccharification of untreated sugarcane biomass, but had no further effect on the pre-treated biomass. Conclusion: Our results show that F. metavorans DSM105788 is a promising alternative pre-treatment for the degradation of agro-industrial lignocellulosic materials. The enzyme cocktail promotes the debranching of biopolymers surrounding the cellulose fibers and releases reduced sugars without process disadvantages or loss of carbohydrates.
546			_aText in English
650		7	_aFusarium _2AGROVOC _92705
650		7	_aGenomes _gAGROVOC _2 _91131
650		7	_aMass spectrometry _2AGROVOC _911789
650		7	_aProteomics _2AGROVOC _918275
650		7	_aCellulose _2AGROVOC _94395
650		7	_aBiomass _2AGROVOC _91897
700	1		_922464 _aBrognaro, H.
700	1		_922465 _aAli, A.
700	1		_922466 _aEllinger, B.
700	1		_922467 _aMaibach, K.
700	1		_922468 _aRühl, M.
700	1		_922469 _aWrenger, C.
700	1		_922470 _aSchlüter, H.
700	1		_922471 _aSchäfer, W.
700	1		_922472 _aBetzel, C.
700	1		_922473 _aJanssen, S.
700	1		_922474 _aGand, M.
773	0		_tBiotechnology for Biofuels _gv. 14, art. 74 _dLondon (United Kingdom) : BioMed Central, 2021. _x1754-6834
856	4		_uhttps://doi.org/10.1186/s13068-021-01927-9 _yClick here to access online
942			_cJA _2ddc _n0