000			03005nab|a22003617a|4500
999			_c64127 _d64119
001			64127
003			MX-TxCIM
005			20211006080707.0
008			200910s2021||||xxk|||p|op||||00||0|eng|d
022			_a1475-2859
024	8		_ahttps://doi.org/10.1186/s12934-021-01540-3
040			_aMX-TxCIM
041			_aeng
100	1		_aKaruppiah, V. _922443
245	1	0	_aCo-culture of Vel1-overexpressed Trichoderma asperellum and Bacillus amyloliquefaciens : _ban eco-friendly strategy to hydrolyze the lignocellulose biomass in soil to enrich the soil fertility, plant growth and disease resistance
260			_aLondon (United Kingdom) : _bBioMed Central, _c2021.
500			_aPeer review
500			_aOpen Access
520			_aBackground: Retention of agricultural bio-mass residues without proper treatment could affect the subsequent plant growth. In the present investigation, the co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens has been employed for multiple benefits including the enrichment of lignocellulose biodegradation, plant growth, defense potential and disease resistance. Results: The Vel1 gene predominantly regulates the secondary metabolites, sexual and asexual development as well as cellulases and polysaccharide hydrolases productions. Overexpression mutant of the Trichoderma asperellum Vel1 locus (TA OE-Vel1) enhanced the activity of FPAase, CMCase, PNPCase, PNPGase, xylanase I, and xylanase II through the regulation of transcription regulating factors and the activation of cellulase and xylanase encoding genes. Further, these genes were induced upon co-cultivation with Bacillus amyloliquefaciens (BA). The co-culture of TA OE-Vel1 + BA produced the best composition of enzymes and the highest biomass hydrolysis yield of 89.56 ± 0.61%. The co-culture of TA OE-Vel1 + BA increased the corn stover degradation by the secretion of cellulolytic enzymes and maintained the C/N ratio of the corn stover amended soil. Moreover, the TA OE-Vel1 + BA increased the maize plant growth, expression of defense gene and disease resistance against Fusarium verticillioides and Cohilohorus herostrophus. Conclusion: The co-cultivation of genetically engineered T. asperellum and B. amyloliquefaciens could be utilized as a profound and meaningful technique for the retention of agro residues and subsequent plant growth.
546			_aText in English
650		7	_aCultivation _gAGROVOC _2 _91071
650		7	_2AGROVOC _922444 _aTrichoderma
650		7	_2AGROVOC _914252 _aBacillus
650		7	_2AGROVOC _912237 _aCellulase
650		7	_2AGROVOC _920039 _aLignocellulose
700	0		_922445 _aLu Zhixiang
700	0		_922446 _aHongyi Liu
700	1		_922447 _aVallikkannu, M.
700	0		_98340 _aJie Chen
773	0		_tMicrobial Cell Factories _gv. 20, art. 57 _dLondon (United Kingdom) : BioMed Central, 2021. _x1475-2859
856	4		_yClick here to access online _uhttps://doi.org/10.1186/s12934-021-01540-3
942			_cJA _n0 _2ddc