000			03062nab|a22003977a|4500
999			_c63952 _d63944
001			63952
003			MX-TxCIM
005			20211006072322.0
008			202112s2021||||xxk|||p|op||||00||0|eng|d
022			_a1474-7596
022			_a1474-760X (Online)
024	8		_ahttps://doi.org/10.1186/s13059-021-02366-3
040			_aMX-TxCIM
041			_aeng
100	1		_aLoudya, N. _921299
245	1	0	_aCellular and transcriptomic analyses reveal two-staged chloroplast biogenesis underpinning photosynthesis build-up in the wheat leaf
260			_aUnited Kingdom : _bBioMed Central, _c2021.
500			_aPeer review
500			_aOpen Access
520			_aBackground: The developmental gradient in monocot leaves has been exploited to uncover leaf developmental gene expression programs and chloroplast biogenesis processes. However, the relationship between the two is barely understood, which limits the value of transcriptome data to understand the process of chloroplast development. Results: Taking advantage of the developmental gradient in the bread wheat leaf, we provide a simultaneous quantitative analysis for the development of mesophyll cells and of chloroplasts as a cellular compartment. This allows us to generate the first biologically-informed gene expression map of this leaf, with the entire developmental gradient from meristematic to fully differentiated cells captured. We show that the first phase of plastid development begins with organelle proliferation, which extends well beyond cell proliferation, and continues with the establishment and then the build-up of the plastid genetic machinery. The second phase is marked by the development of photosynthetic chloroplasts which occupy the available cellular space. Using a network reconstruction algorithm, we predict that known chloroplast gene expression regulators are differentially involved across those developmental stages. Conclusions: Our analysis generates both the first wheat leaf transcriptional map and one of the most comprehensive descriptions to date of the developmental history of chloroplasts in higher plants. It reveals functionally distinct plastid and chloroplast development stages, identifies processes occurring in each of them, and highlights our very limited knowledge of the earliest drivers of plastid biogenesis, while providing a basis for their future identification.
546			_aText in English
650		7	_aWheat _gAGROVOC _2 _91310
650		7	_aPlastids _2AGROVOC _921300
650		7	_aChloroplasts _2AGROVOC _914499
650		7	_aLeaf development _2AGROVOC _914326
700	1		_aMishra, P. _921301
700	0		_aKotaro Takahagi _921302
700	0		_aYukiko Uehara-Yamaguchi _921303
700	0		_aKomaki Inoue _921304
700	1		_aBogre, L. _921305
700	0		_aKeiichi Mochida _921306
700	1		_aLópez-Juez, E. _921307
773	0		_tGenome Biology _gv. 22, art. 151 _dUnited Kingdom : BioMed Central, 2021. _x1474-760X
856	4		_yClick here to access online _uhttps://doi.org/10.1186/s13059-021-02366-3
942			_cJA _n0 _2ddc