000			00595nab|a22002177a|4500
999			_c63884 _d63876
001			63884
003			MX-TxCIM
005			20210629230112.0
008			200423s2021||||xxk|||p|op||||00||0|eng|d
022			_a2045-2322
024	8		_ahttps://doi.org/10.1038/s41598-021-90703-8
040			_aMX-TxCIM
041			_aeng
100	0		_aRuifei Wang _920632
245	1	0	_aAnalysis of methods for quantifying yeast cell concentration in complex lignocellulosic fermentation processes
260			_aLondon (United Kingdom) : _bNature Publishing Group, _c2021.
500			_aPeer review
500			_aOpen Access
520			_aCell mass and viability are tightly linked to the productivity of fermentation processes. In 2nd generation lignocellulose-based media quantitative measurement of cell concentration is challenging because of particles, auto-fluorescence, and intrinsic colour and turbidity of the media. We systematically evaluated several methods for quantifying total and viable yeast cell concentrations to validate their use in lignocellulosic media. Several automated cell counting systems and stain-based viability tests had very limited applicability in such samples. In contrast, manual cell enumeration in a hemocytometer, plating and enumeration of colony forming units, qPCR, and in situ dielectric spectroscopy were further investigated. Parameter optimization to measurements in synthetic lignocellulosic media, which mimicked typical lignocellulosic fermentation conditions, resulted in statistically significant calibration models with good predictive capacity for these four methods. Manual enumeration of cells in a hemocytometer and of CFU were further validated for quantitative assessment of cell numbers in simultaneous saccharification and fermentation experiments on steam-exploded wheat straw. Furthermore, quantitative correlations could be established between these variables and in situ permittivity. In contrast, qPCR quantification suffered from inconsistent DNA extraction from the lignocellulosic slurries. Development of reliable and validated cell quantification methods and understanding their strengths and limitations in lignocellulosic contexts, will enable further development, optimization, and control of lignocellulose-based fermentation processes.
546			_aText in English
650		7	_2AGROVOC _912531 _aFermentation
650		7	_2AGROVOC _916740 _aYeasts
650		7	_2AGROVOC _920633 _aCell counting
700	1		_aLorantfy, B. _920634
700	1		_aFusco, S. _920635
700	1		_aOlsson, L. _920636
700	1		_aFranzén, C.J. _920637
773	0		_tNature Scientific Reports _gv. 11, art. 11293 _dLondon (United Kingdom) : Nature Publishing Group, 2021. _x2045-2322 _wa58025
856	4		_yClick here to access online _uhttps://doi.org/10.1038/s41598-021-90703-8
942			_cJA _n0 _2ddc