000			03060nab|a22004097a|4500
999			_c63877 _d63869
001			63877
003			MX-TxCIM
005			20230313165819.0
008			200910s2021||||xxk|||p|op||||00||0|eng|d
022			_a2049-2618
024	8		_ahttps://doi.org/10.1186/s40168-021-01077-y
040			_aMX-TxCIM
041			_aeng
100	0		_aJing Wang _91646
245	1	0	_aPost-translational regulation of autophagy is involved in intra-microbiome suppression of fungal pathogens
260			_aLondon (United Kingdom) : _bBioMed Central, _c2021.
500			_aPeer review
500			_aOpen Access
520			_aBackground: Microbiome interactions are important determinants for ecosystem functioning, stability, and health. In previous studies, it was often observed that bacteria suppress potentially pathogenic fungal species that are part of the same plant microbiota; however, the underlying microbe-microbe interplay remains mostly elusive. Here, we explored antagonistic interactions of the fungus Fusarium graminearum and bacterium Streptomyces hygroscopicus at the molecular level. Both are ubiquitous members of the healthy wheat microbiota; under dysbiosis, the fungus causes devastating diseases. Results: In co-cultures, we found that Streptomyces alters the fungal acetylome leading to substantial induction of fungal autophagy. The bacterium secrets rapamycin to inactivate the target of rapamycin (TOR), which subsequently promotes the degradation of the fungal histone acetyltransferase Gcn5 through the 26S proteasome. Gcn5 negatively regulates fungal autophagy by acetylating the autophagy-related protein Atg8 at the lysine site K13 and blocking cellular relocalization of Atg8. Thus, degradation of Gcn5 triggered by rapamycin was found to reduce Atg8 acetylation, resulting in autophagy induction in F. graminearum. Conclusions: Autophagy homeostasis plays an essential role in fungal growth and competition, as well as for virulence. Our work reveals a novel post-translational regulation of autophagy initiated by a bacterial antibiotic. Rapamycin was shown to be a powerful modulator of bacteria–fungi interactions with potential importance in explaining microbial homeostasis in healthy plant microbiomes. The autophagic process provides novel possibilities and targets to biologically control pathogens.
546			_aText in English
650		0	_aMicroorganisms _gAGROVOC _910080
650		7	_2AGROVOC _91017 _aBacteria
650		7	_aFungi _gAGROVOC _2 _91121
650		7	_2AGROVOC _92331 _aGibberella zeae
650		7	_2AGROVOC _920578 _aStreptomyces
700	0		_920579 _aChaoyun Xu
700	0		_920580 _aQiming Sun
700	0		_920581 _aJinrong Xu
700	0		_920582 _aYunrong Chai
700	1		_920583 _aBerg, G.
700	1		_920584 _aCernava, T.
700	0		_920585 _aZhonghua Ma
700	0		_920586 _aYun Chen
773	0		_tMicrobiome _gv. 9, art. 131 _dLondon (United Kingdom) : BioMed Central, 2021. _x2049-2618
856	4		_yClick here to access online _uhttps://doi.org/10.1186/s40168-021-01077-y
942			_cJA _n0 _2ddc