000			03349nab|a22003377a|4500
999			_c63875 _d63867
001			63875
003			MX-TxCIM
005			20211006072321.0
008			200910s2021||||xxk|||p|op||||00||0|eng|d
022			_a1471-2229
024	8		_ahttps://doi.org/10.1186/s12870-021-03036-z
040			_aMX-TxCIM
041			_aeng
100	1		_aCalderon Flores, P. _920573
245	1	0	_aEffect of chilling acclimation on germination and seedlings response to cold in different seed coat colored wheat (Triticum aestivum L.)
260			_aLondon (United Kingdom) : _bBioMed Central, _c2021.
500			_aPeer review
500			_aOpen Access
520			_aBackground: Flavonoids can protect plants against extreme temperatures and ROS due to their antioxidant activities. We found that deep-purple seed coat color was controlled by two gene interaction (12:3:1) from the cross between yellow and deep-purple seed coat colored inbreds. F2:3 seeds were grouped in 3 by seed coat color and germinated under chilling (4 °C) and non-acclimated conditions (18 °C) for a week, followed by normal conditions (18 °C) for three weeks and a subsequent chilling stress (4 °C) induction. We analyzed mean daily germination in each group. Additionally, to study the acclimation in relationship to the different seed coat colors on the germination ability and seedling performances under the cold temperatures, we measured the chlorophyll content, ROS scavenging activity, and expression levels of genes involved in ROS scavenging, flavonoid biosynthetic pathway, and cold response in seedlings. Results: The results of seed color segregation between yellow and deep purple suggested a two-gene model. In the germination study, normal environmental conditions induced the germination of yellow-seed, while under chilling conditions, the germination ratio of deep purple-seed was higher than that of yellow-colored seeds. We also found that the darker seed coat colors were highly responsive to cold acclimation based on the ROS scavenging enzymes activity and gene expression of ROS scavenging enzymes, flavonoid biosynthetic pathway and cold responsive genes. Conclusions: We suggest that deep purple colored seed might be in a state of innate pre-acquired stress response state under normal conditions to counteract stresses in a more effective way. Whereas, after the acclimation, another stress should enhance the cold genes expression response, which might result in a more efficient chilling stress response in deep purple seed seedlings. Low temperature has a large impact on the yield of crops. Thus, understanding the benefit of seed coat color response to chilling stress and the identification of limiting factors are useful for developing breeding strategies in order to improve the yield of wheat under chilling stress.
546			_aText in English
650		7	_2AGROVOC _912774 _aCold tolerance
650		7	_2AGROVOC _912884 _aFlavonoids
650		7	_2AGROVOC _915734 _aCold
650		7	_aWheat _gAGROVOC _2 _91310
700	0		_917809 _aJin Seok Yoon
700	0		_917812 _aDae Yeon Kim
700	0		_917813 _aYong Weon Seo
773	0		_tBMC Plant Biology _gv. 21, art. 252 _dLondon (United Kingdom) : BioMed Central, 2021. _x1471-2229 _wGu79387
856	4		_yClick here to access online _uhttps://doi.org/10.1186/s12870-021-03036-z
942			_cJA _n0 _2ddc