000			02956nab|a22003497a|4500
999			_c63826 _d63818
001			63826
003			MX-TxCIM
005			20211006085229.0
008			200910s2021||||xxk|||p|op||||00||0|eng|d
022			_a1471-2229
024	8		_ahttps://doi.org/10.1186/s12870-021-02983-x
040			_aMX-TxCIM
041			_aeng
100	0		_aHuanhuan Liu _920138
245	1	0	_aComparative transcriptome profiling and co-expression network analysis uncover the key genes associated withearly-stage resistance to Aspergillus flavus in maize
260			_aLondon (United Kingdom) : _bBioMed Central, _c2021.
500			_aPeer review
500			_aOpen Access
520			_aBackground: The fungus Aspergillus flavus (A. flavus) is a serious threat to maize (Zea mays) production worldwide. It causes considerable yield and economic losses, and poses a health risk to humans and livestock due to the high toxicity of aflatoxin. However, key genes and regulatory networks conferring maize resistance to A. flavus are not clear, especially at the early stage of infection. Here, we performed a comprehensive transcriptome analysis of two maize inbred lines with contrasting resistance to A. flavus infection. Results: The pairwise comparisons between mock and infected kernels in each line during the first 6 h post inoculation (hpi) showed that maize resistance to A. flavus infection was specific to the genotype and infection stage, and defense pathways were strengthened in the resistant line. Further comparison of the two maize lines revealed that the infection-induced up-regulated differentially expressed genes (DEGs) in the resistant line might underlie the enhanced resistance. Gene co-expression network analysis by WGCNA (weighted gene co-expression network analysis) identified 7 modules that were significantly associated with different infection stages, and 110 hub genes of these modules. These key regulators mainly participate in the biosynthesis of fatty acid and antibiotics. In addition, 90 candidate genes for maize resistance to A. flavus infection and/or aflatoxin contamination obtained in previous studies were confirmed to be differentially expressed between the resistant and susceptible lines within the first 6 hpi. Conclusion: This work unveiled more A. flavus resistance genes and provided a detailed regulatory network of early-stage resistance to A. flavus in maize.
546			_aText in English
650		7	_aMaize _gAGROVOC _2 _91173
650		7	_2AGROVOC _95636 _aAspergillus flavus
650		7	_2AGROVOC _920139 _aTranscriptome
700	0		_920140 _aHaofeng Wu
700	0		_920141 _aYan Wang
700	0		_920142 _aHuan Wang
700	0		_920143 _aSaihua Chen
700	0		_920144 _aZhitong Yin
773	0		_tBMC Plant Biology _gv. 21, art. 216 _dLondon (United Kingdom) : BioMed Central, 2021. _x1471-2229 _wGu79387
856	4		_yClick here to access online _uhttps://doi.org/10.1186/s12870-021-02983-x
942			_cJA _n0 _2ddc