000			02894nab|a22003257a|4500
999			_c63676 _d63668
001			63676
003			MX-TxCIM
005			20230313165819.0
008			201209s2021||||xxk|||p|op||||00||0|eng|d
022			_a2045-2322
024	8		_ahttps://doi.org/10.1038/s41598-021-83497-2
040			_aMX-TxCIM
041			_aeng
100	1		_aMason, C.J. _919795
245	1	0	_aEffects of maize (Zea mays) genotypes and microbial sources in shaping fall armyworm (Spodoptera frugiperda) gut bacterial communities
260			_aLondon (United Kingdom) : _bNature Publishing Group, _c2021.
500			_aPeer review
500			_aOpen Access
520			_aPlants can have fundamental roles in shaping bacterial communities associated with insect herbivores. For larval lepidopterans (caterpillars), diet has been shown to be a driving force shaping gut microbial communities, where the gut microbiome of insects feeding on different plant species and genotypes can vary in composition and diversity. In this study, we aimed to better understand the roles of plant genotypes, sources of microbiota, and the host gut environment in structuring bacterial communities. We used multiple maize genotypes and fall armyworm (Spodoptera frugiperda) larvae as models to parse these drivers. We performed a series of experiments using axenic larvae that received a mixed microbial community prepared from frass from larvae that consumed field-grown maize. The new larval recipients were then provided different maize genotypes that were gamma-irradiated to minimize bacteria coming from the plant during feeding. For field-collected maize, there were no differences in community structure, but we did observe differences in gut community membership. In the controlled experiment, the microbial inoculation source, plant genotype, and their interactions impacted the membership and structure of gut bacterial communities. Compared to axenic larvae, fall armyworm larvae that received frass inoculum experienced reduced growth. Our results document the role of microbial sources and plant genotypes in contributing to variation in gut bacterial communities in herbivorous larvae. While more research is needed to shed light on the mechanisms driving this variation, these results provide a method for incorporating greater gut bacterial community complexity into laboratory-reared larvae.
546			_aText in English
650		7	_aMaize _gAGROVOC _2 _91173
650		7	_aGenotypes _2AGROVOC _91134
650		0	_aMicroorganisms _gAGROVOC _910080
650		7	_aSpodoptera frugiperda _2AGROVOC _96410
700	1		_aHoover, K. _919796
700	1		_aFelton, G.W. _919797
773	0		_gv. 11, art. 4429 _dLondon (United Kingdom) : Nature Publishing Group, 2021. _x2045-2322 _tNature Scientific Reports _wa58025
856	4		_yClick here to access online _uhttps://doi.org/10.1038/s41598-021-83497-2
942			_cJA _n0 _2ddc