000			03628nab a22003737a 4500
999			_c63492 _d63484
001			63492
003			MX-TxCIM
005			20211006085226.0
008			202003s2020 sz |||p|op||| 00| 0 eng d
022			_a1664-462X
024	8		_ahttps://doi.org/10.3389/fpls.2020.00191
040			_aMX-TxCIM
041			_aeng
100	1		_aWelgemoed, T. _919154
245	1	0	_aDe novo assembly of transcriptomes from a B73 maize line introgressed with a QTL for resistance to gray leaf spot disease reveals a candidate allele of a lectin receptor-like kinase
260			_aSwitzerland : _bFrontiers, _c2020.
500			_aPeer review
500			_aOpen Access
520			_aGray leaf spot (GLS) disease in maize, caused by the fungus Cercospora zeina, is a threat to maize production globally. Understanding the molecular basis for quantitative resistance to GLS is therefore important for food security. We developed a de novo assembly pipeline to identify candidate maize resistance genes. Near-isogenic maize lines with and without a QTL for GLS resistance on chromosome 10 from inbred CML444 were produced in the inbred B73 background. The B73-QTL line showed a 20% reduction in GLS disease symptoms compared to B73 in the field (p = 0.01). B73-QTL leaf samples from this field experiment conducted under GLS disease pressure were RNA sequenced. The reads that did not map to the B73 or C. zeina genomes were expected to contain novel defense genes and were de novo assembled. A total of 141 protein-coding sequences with B73-like or plant annotations were identified from the B73-QTL plants exposed to C. zeina. To determine whether candidate gene expression was induced by C. zeina, the RNAseq reads from C. zeina-challenged and control leaves were mapped to a master assembly of all of the B73-QTL reads, and differential gene expression analysis was conducted. Combining results from both bioinformatics approaches led to the identification of a likely candidate gene, which was a novel allele of a lectin receptor-like kinase named L-RLK-CML that (i) was induced by C. zeina, (ii) was positioned in the QTL region, and (iii) had functional domains for pathogen perception and defense signal transduction. The 817AA L-RLK-CML protein had 53 amino acid differences from its 818AA counterpart in B73. A second “B73-like” allele of L-RLK was expressed at a low level in B73-QTL. Gene copy-specific RT-qPCR confirmed that the l-rlk-cml transcript was the major product induced four-fold by C. zeina. Several other expressed defense-related candidates were identified, including a wall-associated kinase, two glutathione s-transferases, a chitinase, a glucan beta-glucosidase, a plasmodesmata callose-binding protein, several other receptor-like kinases, and components of calcium signaling, vesicular trafficking, and ethylene biosynthesis. This work presents a bioinformatics protocol for gene discovery from de novo assembled transcriptomes and identifies candidate quantitative resistance genes.
546			_aText in English
650		7	_aMaize _gAGROVOC _2 _91173
650		7	_2AGROVOC _919155 _aCercospora
650		7	_2AGROVOC _96337 _aLeaf spots
650		7	_2AGROVOC _912820 _aKinases
700	1		_aPierneef, R. _919156
700	1		_aSterck, L. _919157
700	1		_aVan de Peer, Y. _919158
700	1		_aSwart, V. _919159
700	1		_aScheepers, K.D. _919160
700	1		_aBerger, D.K. _918997
773	0		_tFrontiers in Plant Science _gv. 11, art. 191 _dSwitzerland : Frontiers, 2020. _x1664-462X _wu56875
856	4		_yClick here to access online _uhttps://doi.org/10.3389/fpls.2020.00191
942			_cJA _n0 _2ddc