000			02653nab|a22003737a|4500
999			_c63361 _d63353
001			63361
003			MX-TxCIM
005			20211006085223.0
008			201209s2021||||xxk|||p|op||||00||0|eng|d
022			_a2045-2322
024	8		_ahttps://doi.org/10.1038/s41598-020-79320-z
040			_aMX-TxCIM
041			_aeng
100	1		_aMurphy, K.M. _918592
245	1	0	_aBioactive diterpenoids impact the composition of the root-associated microbiome in maize (Zea mays)
260			_aLondon (United Kingdom) : _bNature Publishing Group, _c2021.
500			_aPeer review
500			_aOpen Access
520			_aPlants deploy both primary and species-specific, specialized metabolites to communicate with other organisms and adapt to environmental challenges, including interactions with soil-dwelling microbial communities. However, the role of specialized metabolites in modulating plant-microbiome interactions often remains elusive. In this study, we report that maize (Zea mays) diterpenoid metabolites with known antifungal bioactivities also influence rhizosphere bacterial communities. Metabolite profiling showed that dolabralexins, antibiotic diterpenoids that are highly abundant in roots of some maize varieties, can be exuded from the roots. Comparative 16S rRNA gene sequencing determined the bacterial community composition of the maize mutant Zman2 (anther ear 2), which is deficient in dolabralexins and closely related bioactive kauralexin diterpenoids. The Zman2 rhizosphere microbiome differed significantly from the wild-type sibling with the most significant changes observed for Alphaproteobacteria of the order Sphingomonadales. Metabolomics analyses support that these differences are attributed to the diterpenoid deficiency of the Zman2 mutant, rather than other large-scale metabolome alterations. Together, these findings support physiological functions of maize diterpenoids beyond known chemical defenses, including the assembly of the rhizosphere microbiome.
546			_aText in English
650		7	_aSoil microorganisms _2AGROVOC _97739
650		7	_aRoots _2AGROVOC _91755
650		7	_aMaize _gAGROVOC _2 _91173
650		7	_aDiterpenoids _2AGROVOC _918593
700	1		_aEdwards, J. _918594
700	1		_aLouie, K.B. _918595
700	1		_aBowen, B.P. _918596
700	1		_aSundaresan, V. _918597
700	1		_aNorthen, T.R. _918598
700	1		_aZerbe, P. _918599
773	0		_gv. 11, art. 333 _dLondon (United Kingdom) : Nature Publishing Group, 2021. _x2045-2322 _tNature Scientific Reports _wa58025
856	4		_yClick here to access online _uhttps://doi.org/10.1038/s41598-020-79320-z
942			_cJA _n0 _2ddc