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003			MX-TxCIM
005			20211014165440.0
008			200910s2021||||xxk|||p|op||||00||0|eng|d
022			_a1471-2229
024	8		_ahttps://doi.org/10.1186/s12870-020-02806-5
040			_aMX-TxCIM
041			_aeng
100	0		_aDaoping Wang _918532
245	1	0	_aComparative proteomic analysis reveals that the Heterosis of two maize hybrids is related to enhancement of stress response and photosynthesis respectively
260			_aLondon (United Kingdom) : _bBioMed Central, _c2021.
500			_aPeer review
500			_aOpen Access
520			_aBackground: Heterosis refers to superior traits exhibiting in a hybrid when compared with both parents. Generally, the hybridization between parents can change the expression pattern of some proteins such as non-additive proteins (NAPs) which might lead to heterosis. ‘Zhongdan808’ (ZD808) and ‘Zhongdan909’ (ZD909) are excellent maize hybrids in China, however, the heterosis mechanism of them are not clear. Proteomics has been wildly used in many filed, and comparative proteomic analysis of hybrid and its parents is helpful for understanding the mechanism of heterosis in the two maize hybrids. Results: Over 2000 protein groups were quantitatively identified from second seedling leaves of two hybrids and their parents by label-free quantification. Statistical analysis of total identified proteins, differentially accumulated proteins (DAPs) and NAPs of the two hybrids revealed that both of them were more similar to their female parents. In addition, most of DAPs were up-regulated and most of NAPs were high parent abundance or above-high parent abundance in ZD808, while in ZD909, most of DAPs were down-regulated and most of NAPs were low parent abundance or below-low parent abundance. Pathway enrichment analysis showed that more of stress response-related NAPs in ZD808 were high parent abundance or above-high parent abundance, and most of PS related NAPs in ZD909 were high parent abundance or above-high parent abundance. Finally, four stress response-related proteins and eight proteins related to PS were verified by PRM, ten of them had significant differences between hybrid and midparent value. Conclusions: Even though every one of the two hybrids were more similar to its female parent at proteome level, the biological basis of heterosis is different in the two maize hybrids. In comparison with their parents, the excellent agronomic traits of hybrid ZD808 is mainly correlated with the high expression levels of some proteins related to stress responses and metabolic functions, while traits of ZD909 is mainly correlated with high expressed proteins related to photosynthesis. Our proteomics results support previous physiological and morphological research and have provided useful information in understanding the reason of valuable agronomic traits.
546			_aText in English
650		7	_2AGROVOC _91145 _aHeterosis
650		7	_2AGROVOC _91224 _aProteins
650		7	_2AGROVOC _918275 _aProteomics
650		7	_aMaize _2AGROVOC _91173
700	0		_918533 _aYongying Mu
700	0		_918534 _aXiaojiao Hu
700	0		_918535 _aBo Ma
700	0		_918536 _aZhibo Wang
700	0		_918537 _aLi Zhu
700	0		_918538 _aJiang Xu
700	0		_92731 _aChangling Huang
700	0		_918539 _aYinghong Pan
773	0		_tBMC Plant Biology _gv. 21, art. 34 _dLondon (United Kingdom) : BioMed Central, 2021. _x1471-2229 _wGu79387
856	4		_yClick here to access online _uhttps://doi.org/10.1186/s12870-020-02806-5
942			_cJA _n0 _2ddc
999			_c63353 _d63345