000 nab a22 7a 4500
999 _c63103
_d63095
001 63103
003 MX-TxCIM
005 20210122214710.0
008 202102s2020||||ne |||p|op||||00||0|eng|d
022 _a2452-2198
024 8 _ahttps://doi.org/10.1016/j.rhisph.2020.100260
040 _aMX-TxCIM
041 _aeng
100 1 _aAhangir, A.
_917876
245 1 0 _aDrought tolerant maize cultivar accumulates putrescine in roots
260 _aNetherlands :
_bElsevier,
_c2020.
500 _aPeer review
520 _aWe compared putrescine metabolism in roots of tolerant maize cultivar (Karoon) to a susceptible cultivar (260) under drought stress. The experiment was conducted in growth chamber under controlled condition in 80 pots. Drought stress led to significant increase in root growth indices, H2O2 content and activity of antioxidant enzymes (superoxide dismutase, catalase, and peroxidase). The qPCR analysis revealed that expression of maize polyamine oxidase (ZmPAO) genes including ZmPAO1, ZmPAO2, ZmPAO4 and ZmPAO6 as well as ornithine decarboxylase (ZmODC) significantly increased in root of both cultivars in response to drought. However, up-regulation of ZmPAO3 and ZmPAO5 was only observed in root of tolerant cultivar. Consistently, drought caused increased polyamine oxidase activity in the tolerant cultivar. Transcription of spermidine synthase (ZmSPDS) was down-regulated by drought stress. Transcript levels of ZmPOA, ZmODC and ZmSPDS were more affected by drought in tolerant cultivar compared to susceptible cultivar. Increase in putrescine content under drought stress was more prominent in roots of tolerant cultivar, compared to susceptible cultivar. We attribute the accumulation of putrescine to increased rate of putrescine synthesis, due to higher expression of ZmPAOs and ZmODC and decreased rate of its depletion for biosynthesis of higher polyamines due to down-regulation of ZmSPDS.
546 _aText in English
650 7 _aAbiotic stress
_2AGROVOC
_93448
650 7 _aAntioxidants
_2AGROVOC
_911020
650 7 _aOxidative stress
_2AGROVOC
_911815
650 7 _aPolyamines
_2AGROVOC
_917877
650 7 _aDrought stress
_2AGROVOC
_91081
700 1 _aGhotbi-Ravandi, A.A.
_917878
700 1 _aRezadoost, H.
_917879
700 1 _aBernard, F.
_917880
773 0 _tRhizosphere
_gv. 16, art. 100260
_dNetherlands : Elsevier, 2020.
_x2452-2198
942 _cJA
_n0
_2ddc