000 02494nab|a22003377a|4500
999 _c63091
_d63083
001 63091
003 MX-TxCIM
005 20211006072314.0
008 210113s2020||||ne |||p|op||||00||0|eng|d
022 _a0981-9428
024 8 _ahttps://doi.org/10.1016/j.plaphy.2020.11.014
040 _aMX-TxCIM
041 _aeng
100 0 _aJin Seok Yoon
_917809
245 1 2 _aA novel wheat ASR gene, TaASR2D, enhances drought tolerance in Brachypodium distachyon
260 _aNetherlands :
_bElsevier,
_c2020.
500 _aPeer review
520 _aAbscisic acid-, stress-, and ripening-induced (ASR) proteins play an important role in protecting plants against adverse environmental conditions. Here, we identified 24 ASR genes in the wheat genome and analyzed their characteristics. Among these, five ASR genes highly induced by abscisic acid (ABA) and polyethylene glycol were cloned and further characterized. The TaASR genes were expressed in response to different abiotic stresses and ABA and were found to be localized in the nucleus and plasma membrane of transformed tobacco cells. Brachypodium distachyon transgenic plants overexpressing TaASR2D showed enhanced drought tolerance by regulating leaf transpiration. The expression levels of stress-related and ABA-responsive genes were higher in transgenic plants than in wild-type plants under drought stress conditions. Moreover, overexpression of TaASR2D increased the levels of both endogenous ABA and hydrogen peroxide in response to drought stress, and these plants showed hypersensitivity to exogenous ABA at the germination stage. Furthermore, plants overexpressing TaASR2D showed increased stomatal closure. Further analysis revealed that TaASR2D interacts with ABA biosynthesis and stress-related proteins in yeast and tobacco plants. Collectively, these findings indicate that TaASR2D plays an important role in the response of plants to drought stress by regulating the ABA biosynthesis pathway and redox homeostasis system.
546 _aText in English
650 7 _aWheat
_gAGROVOC
_2
_91310
650 7 _aDrought
_2AGROVOC
_91080
650 7 _aABA
_2AGROVOC
_93517
650 7 _aProteins
_2AGROVOC
_91224
650 7 _aStomata
_2AGROVOC
_917810
650 7 _aGenes
_2AGROVOC
_93563
700 0 _aJae Yoon Kim
_917811
700 0 _aDae Yeon Kim
_917812
700 0 _aYong Weon Seo
_917813
773 0 _gIn press
_dNetherlands : Elsevier, 2020.
_x0981-9428
_tPlant Physiology and Biochemistry
_wu91708
942 _cJA
_n0
_2ddc