| 000 | 03862nab|a22004577a|4500 | ||
|---|---|---|---|
| 999 |
_c63038 _d63030 |
||
| 001 | 63038 | ||
| 003 | MX-TxCIM | ||
| 005 | 20211006080209.0 | ||
| 008 | 201222s2020 sz |||p|op||| 00| 0 eng d | ||
| 022 | _a1664-8021 (Online) | ||
| 024 | 8 | _ahttps://doi.org/10.3389/fgene.2020.00638 | |
| 040 | _aMX-TxCIM | ||
| 041 | _aeng | ||
| 100 | 0 |
_aZhiyong Li _917645 |
|
| 245 | 1 | 1 | _aCharacterization of genetic diversity and genome-wide association mapping of three agronomic traits in Qingke barley (Hordeum Vulgare L.) in the Qinghai-Tibet Plateau |
| 260 |
_aSwitzerland : _bFrontiers, _c2020. |
||
| 500 | _aPeer review | ||
| 500 | _aOpen Access | ||
| 520 | _aBarley (Hordeum vulgare L.) is one of the most important cereal crops worldwide. In the Qinghai-Tibet Plateau, six-rowed hulless (or naked) barley, called “qingke” in Chinese or “nas” in Tibetan, is produced mainly in Tibet. The complexity of the environment in the Qinghai-Tibet Plateau has provided unique opportunities for research on the breeding and adaptability of qingke barley. However, the genetic architecture of many important agronomic traits for qingke barley remains elusive. Heading date (HD), plant height (PH), and spike length (SL) are three prominent agronomic traits in barley. Here, we used genome-wide association (GWAS) mapping and GWAS with eigenvector decomposition (EigenGWAS) to detect quantitative trait loci (QTL) and selective signatures for HD, PH, and SL in a collection of 308 qingke barley accessions. The accessions were genotyped using a newly-developed, proprietary genotyping-by-sequencing (tGBS) technology, that yielded 14,970 high quality single nucleotide polymorphisms (SNPs). We found that the number of SNPs was higher in the varieties than in the landraces, which suggested that Tibetan varieties and varieties in the Tibetan area may have originated from different landraces in different areas. We have identified 62 QTLs associated with three important traits, and the observed phenotypic variation is well-explained by the identified QTLs. We mapped 114 known genes that include, but are not limited to, vernalization, and photoperiod genes. We found that 83.87% of the identified QTLs are located in the non-coding regulatory regions of annotated barley genes. Forty-eight of the QTLs are first reported here, 28 QTLs have pleotropic effects, and three QTL are located in the regions of the well-characterized genes HvVRN1, HvVRN3, and PpD-H2. EigenGWAS analysis revealed that multiple heading-date-related loci bear signatures of selection. Our results confirm that the barley panel used in this study is highly diverse, and showed a great promise for identifying the genetic basis of adaptive traits. This study should increase our understanding of complex traits in qingke barley, and should facilitate genome-assisted breeding for qingke barley improvement. | ||
| 546 | _aText in English | ||
| 591 | _aJiankang Wang : No CIMMYT Affiliation | ||
| 650 | 7 |
_aBarley _2AGROVOC _91018 |
|
| 650 | 7 |
_aHordeum vulgare _2AGROVOC _91149 |
|
| 650 | 7 |
_aGenetic diversity _2AGROVOC _91125 |
|
| 650 | 7 |
_aAdaptation _2AGROVOC _96026 |
|
| 650 | 7 |
_aAgronomic characters _gAGROVOC _2 _91008 |
|
| 700 | 1 |
_aLhundrup, N. _917646 |
|
| 700 | 0 |
_aGanggang Guo _917647 |
|
| 700 | 1 |
_aDol, K. _917648 |
|
| 700 | 0 |
_aPanpan Chen _917649 |
|
| 700 | 0 |
_aLiyun Gao _917650 |
|
| 700 | 0 |
_aWangmo Chemi _917651 |
|
| 700 | 0 |
_aJing Zhang _917652 |
|
| 700 | 1 |
_aJiankang Wang _8INT2542 _9842 _gGenetic Resources Program |
|
| 700 | 0 |
_aTashi Nyema _917653 |
|
| 700 | 1 |
_aDondrup Dawa _917654 |
|
| 700 | 1 |
_aHuihui Li _8CLIH01 _9764 _gGenetic Resources Program |
|
| 773 | 0 |
_tFrontiers in Genetics _gv. 11, art. 638 _dSwitzerland : Frontiers, 2020. _x1664-8021 |
|
| 856 | 4 |
_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/21089 |
|
| 942 |
_cJA _n0 _2ddc |
||