| 000 | 03774nab|a22004817a|4500 | ||
|---|---|---|---|
| 999 |
_c62940 _d62932 |
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| 001 | 62940 | ||
| 003 | MX-TxCIM | ||
| 005 | 20231018183733.0 | ||
| 008 | 201118s2020||||xxu|||p|op||||00||0|eng|d | ||
| 022 | _a1932-6203 (Online) | ||
| 024 | 8 | _ahttps://doi.org/10.1371/journal.pone.0238724 | |
| 040 | _aMX-TxCIM | ||
| 041 | _aeng | ||
| 100 | 1 |
_aTembo, B. _917264 |
|
| 245 | 1 | 0 | _aDetection and characterization of fungus (Magnaporthe oryzae pathotype Triticum) causing wheat blast disease on rain-fed grown wheat (Triticum aestivum L.) in Zambia |
| 260 |
_aSan Francisco, CA (USA) : _bPublic Library of Science, _c2020. |
||
| 500 | _aPeer review | ||
| 500 | _aOpen Access | ||
| 520 | _aWheat blast caused by Magnaporthe oryzae pathotype Triticum (MoT) is a threat to wheat production especially in the warmer-humid environments. In Zambia, wheat blast symptoms were observed for the first time on wheat (Triticum aestivum L.) grown in experimental plots and five farmers’ fields in Mpika district of Muchinga Province during the 2017–18 rainy season. Infected plants showed the typical wheat blast symptoms with the spike becoming partially or completely bleached with the blackening of the rachis in a short span of time. Incidence of blast symptoms on nearly all wheat heads was high and ranged from 50 to 100%. Examination of diseased plant leaves showed the presence of elliptical, grayish to tan necrotic lesions with dark borders on the leaf often mixed with other foliar diseases. A study was conducted to isolate and identify the causal pathogen(s) using classical and molecular methods and determine the pathogenicity of the detected disease causal agent. Morphobiometrical determination of causal pathogen revealed conidia with characteristic pear shaped 2-septate hyaline spores associated with M. oryzae species. Preliminary polymerase chain reaction screening of six isolates obtained from wheat blast infected samples with diagnostic primers (MoT3F/R) was conducted at ZARI, Zambia, and subsequent analysis of two isolates with MoT3F/R and C17F/R was performed at USDA-ARS, USA. Both experiments confirmed that MoT is the causal agent of wheat blast in Zambia. Further, pathogenicity tests performed with pure culture isolates from samples WS4 and WS5 produced typical blast symptoms on all the six inoculated wheat genotypes. Results of this study indicate that MoT is causing wheat blast in rain-fed wheat grown in Zambia, thus making it the first report of MoT in Zambia and Africa. This inter-continental movement of the pathogen (disease) has serious implication for wheat production and trade that needs to be urgently addressed. | ||
| 546 | _aText in English | ||
| 650 | 7 |
_aWheat _gAGROVOC _2 _91310 |
|
| 650 | 7 |
_aPCR _2AGROVOC _912563 |
|
| 650 | 7 |
_aFungi _gAGROVOC _2 _91121 |
|
| 650 | 7 |
_aEpidemiology _2AGROVOC _96627 |
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| 650 | 7 |
_aFungal spores _2AGROVOC _917265 |
|
| 650 | 7 |
_aPathogenesis _2AGROVOC _94080 |
|
| 650 | 7 |
_aLeaves _2AGROVOC _98838 |
|
| 651 | 7 |
_2AGROVOC _94309 _aZambia |
|
| 700 | 1 |
_aRabson M. Mulenga _913646 |
|
| 700 | 1 |
_aSichilima, S. _917266 |
|
| 700 | 1 |
_aM’siska, K.K. _917267 |
|
| 700 | 1 |
_aMwale, M. _917268 |
|
| 700 | 1 |
_aChikoti, P.C. _917269 |
|
| 700 | 1 |
_aPawan Kumar Singh _gGlobal Wheat Program _8INT2868 _9868 |
|
| 700 | 1 |
_aXinyao He _9913 _8INT3297 _gGlobal Wheat Program |
|
| 700 | 1 |
_aPedley, K.F. _94214 |
|
| 700 | 1 |
_aPeterson, G.L. _94213 |
|
| 700 | 1 |
_aSingh, R.P. _gGlobal Wheat Program _8INT0610 _9825 |
|
| 700 | 1 |
_aBraun, H.J. _gFormerly Global Wheat Program _8INT0599 _9824 |
|
| 773 | 0 |
_gv. 15, no. 9, e0238724 _dSan Francisco, CA (USA) : Public Library of Science, 2020. _tPLoS One _x1932-6203 _wu94957 |
|
| 856 | 4 |
_yOpen Access through DSpace _uhttps://hdl.handle.net/10883/21036 |
|
| 942 |
_cJA _n0 _2ddc |
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