000			00595nab|a22002177a|4500
999			_c62620 _d62612
001			62620
003			MX-TxCIM
005			20200928184447.0
008			200910s2019||||xxk|||p|op||||00||0|eng|d
022			_a0022-0957
022			_a1460-2431 (Online)
024	8		_ahttps://doi.org/10.1093/jxb/erz344
040			_aMX-TxCIM
041			_aeng
100	0		_aHiromi Suzuki _916088
245	1	0	_aLow-fluence blue light-induced phosphorylation of Zmphot1 mediates the first positive phototropism
260			_aOxford (United Kingdom) : _bOxford University Press, _c2019.
500			_aPeer review
520			_aPhototropin1 (phot1) perceives low- to high-fluence blue light stimuli and mediates both the first and second positive phototropisms. High-fluence blue light is known to induce autophosphorylation of phot1, leading to the second positive phototropism. However, the phosphorylation status of phot1 by low-fluence blue light that induces the first positive phototropism had not been observed. Here, we conducted a phosphoproteomic analysis of maize coleoptiles to investigate the fluence-dependent phosphorylation status of Zmphot1. High-fluence blue light induced phosphorylation of Zmphot1 at several sites. Notably, low-fluence blue light significantly increased the phosphorylation level of Ser291 in Zmphot1. Furthermore, Ser291-phosphorylated and Ser369Ser376-diphosphorylated peptides were found to be more abundant in the low-fluence blue light-irradiated sides than in the shaded sides of coleoptiles. The roles of these phosphorylation events in phototropism were explored by heterologous expression of ZmPHOT1 in the Arabidopsis thaliana phot1phot2 mutant. The first positive phototropism was restored in wild-type ZmPHOT1-expressing plants; however, plants expressing S291A-ZmPHOT1 or S369AS376A-ZmPHOT1 showed significantly reduced complementation rates. All transgenic plants tested in this study exhibited a normal second positive phototropism. These findings provide the first indication that low-fluence blue light induces phosphorylation of Zmphot1 and that this induced phosphorylation is crucial for the first positive phototropism.
546			_aText in English
650		7	_2AGROVOC _93125 _aArabidopsis thaliana
650		7	_2AGROVOC _912363 _aColeoptiles
650		7	_2AGROVOC _91314 _aZea mays
700	0		_916089 _aTomokazu Koshiba
700	0		_916090 _aChiharu Fujita
700	0		_916091 _aYoshio Yamauchi
700	0		_916092 _aTaro Kimura
700	0		_916093 _aToshiaki Isobe
700	0		_916094 _aTatsuya Sakai
700	0		_916095 _aMasato Taoka
700	0		_916096 _aTakashi Okamoto
773	0		_tJournal of Experimental Botany _gv. 70, no. 20, p. 5929-5941 _dOxford (United Kingdom) : Oxford University Press, 2019. _x0022-0957 _wu444540
942			_cJA _n0 _2ddc