000			nab a22 7a 4500
999			_c62556 _d62548
001			62556
003			MX-TxCIM
005			20200911205319.0
008			200602s2020 ne |||p|op||| 00| 0 eng d
022			_a0981-9428
024	8		_ahttps://doi.org/10.1016/j.plaphy.2020.07.031
040			_aMX-TxCIM
041			_aeng
100	1		_915763 _aMeena, R.P.
245	1	0	_aNovel ASR isolated from drought stress responsive SSH library in pearl millet confers multiple abiotic stress tolerance in PgASR3 transgenic Arabidopsis
260			_aAmsterdam (Netherlands) : _bElsevier, _c2020.
500			_aPeer review
520			_aA genomic resource of drought stress responsive genes/ESTs was generated using Suppression Subtractive Hybridization (SSH) approach in a drought stress tolerant Pennisetum glaucum genotype 841B. Fifty five days old plants were subjected to drought stress after withholding water for different time intervals (10 days, 15 days, 20 days and 25 days). A forward subtractive cDNA library was prepared from isolated RNA of leaf tissue. Differential gene expression under drought stress was validated for selected nine contigs by RT-qPCR. A transcript homologous to Setaria italica ASR3 upregulated under drought stress was isolated from genotype 841B and characterized. Heterologous expression of PgASR3 was validated in Arabidopsis and confirmed under multiple abiotic stress conditions. A total of four independent transgenic lines overexpressing gene PgASR3 were analyzed by Southern blot at T1 stage. For drought stress tolerance, three independent lines (T2 stage) were analyzed by biochemical and physiological assays at seedling stage. The growth rate (shoot and root length) of transgenic seedlings improved as compared to WT seedling under differenct abiotic stress conditions. The three transgenic lines were also validated for drought stress tolerance and RT-qPCR analysis, at maturity stage. Under drought stress conditions, the mature transgenic lines showed higher levels of RWC, chlorophyll and proline but lower levels of MDA as compared to WT plants. PgASR3 gene isolated and validated in this study can be utilized for developing abiotic stress tolerant crops.
546			_aText in English
650		7	_2AGROVOC _93448 _aAbiotic stress
650		7	_2AGROVOC _91081 _aDrought stress
650		7	_2AGROVOC _92433 _aPennisetum glaucum
650		7	_2AGROVOC _915764 _aTransgenics
700	1		_915765 _aVishwakarma, H.
700	1		_915766 _aGhosh, G.
700	1		_915767 _aGaikwad, K.
700	1		_915768 _aChellapilla, T.S.
700	1		_915769 _aSingh, M.P
700	1		_915770 _aPadaria, J.C.
773	0		_dAmsterdam (Netherlands) : Elsevier, 2020. _gv. 156, p. 7-19 _tPlant Physiology and Biochemistry _x0981-9428 _wu91708
942			_2ddc _cJA _n0