| 000 | 02465nab a22003377a 4500 | ||
|---|---|---|---|
| 999 |
_c62508 _d62500 |
||
| 001 | 62508 | ||
| 003 | MX-TxCIM | ||
| 005 | 20211006072308.0 | ||
| 008 | 200212s2020 ie |||p|op||| 00| 0 eng d | ||
| 022 | _a0168-9452 | ||
| 024 | 8 | _ahttps://doi.org/10.1016/j.plantsci.2020.110613 | |
| 040 | _aMX-TxCIM | ||
| 041 | _aeng | ||
| 100 | 0 |
_914078 _aXiaorui Li |
|
| 245 | 1 | 0 | _aA wheat R2R3 MYB gene TaMpc1-D4 negatively regulates drought tolerance in transgenic Arabidopsis and wheat |
| 260 |
_aLimerick (Ireland) : _bElsevier, _c2020. |
||
| 500 | _aPeer review | ||
| 520 | _aMYB transcription factors (TFs) are one of the largest TF families, and R2R3-type MYB TFs participate in the multiply abiotic stress responses in wheat. In this study, an R2R3-type MYB gene Myb protein colourless 1 located on chromosome D (named TaMpc1-D4), was cloned from wheat. TaMpc1-D4-GFP protein was localized in the nucleus. Overexpression of TaMpc1-D4 reduced drought tolerance in transgenic Arabidopsis lines, which was supported by the lower germination rate, the shorter root length, a higher level of O2− and malonaldehyde (MDA), the decreased proline content, and limited activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT). Furthermore, P5CS1, RD29A, RD29B, DREB2A, ABF3, CBF1, CBF2, CBF3, ERF1, POD1, SOD (Cu/Zn), and CAT1 genes related to the stress and antioxidant system were remarkably down-regulated in TaMpc1-D4 transgenic Arabidopsis lines under drought stress. Silencing TaMpc1-D4 expression in wheat enhanced the relative water content (RWC), the proline content, and the activities of antioxidant enzymes, and activated stress-related and antioxidant-related genes (DREB1, DREB3, ERF3, ERF4b, ABF, P5CS, POD, SOD (Fe), and CAT). Taken together, these results indicated that TaMpc1-D4 negatively modulated drought tolerance by regulating the capacity of the enzyme system and the expression of stress-related and antioxidant-related genes. | ||
| 546 | _aText in English | ||
| 650 | 7 |
_aWheat _gAGROVOC _2 _91310 |
|
| 650 | 7 |
_2AGROVOC _96527 _aTranscription factors |
|
| 650 | 7 |
_2AGROVOC _91081 _aDrought stress |
|
| 700 | 0 |
_914080 _aYan Tang |
|
| 700 | 0 |
_915593 _aHailan Li |
|
| 700 | 0 |
_915594 _aWen Luo |
|
| 700 | 0 |
_914081 _aChunju Zhou |
|
| 700 | 0 |
_914082 _aLixin Zhang |
|
| 700 | 0 |
_914083 _aJinyin Lv |
|
| 773 | 0 |
_dLimerick (Ireland) : Elsevier, 2020. _gv. 299, art. 110613 _tPlant Science _x0168-9452 _wu444702 |
|
| 942 |
_2ddc _cJA _n0 |
||